PCF11 Antibody
Catalog Number: LS-C781545-100
100 µL / $365.00
Antibody: Rabbit Polyclonal
Applications: Enzyme-Linked Immunosorbent Assay, Western Blot
Reactivity: Human
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human PCF11 Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$153.00each
Catalog Number: LS-G80622-20
Catalog Number: LS-G80622
For bulk orders or custom formulations:
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human PCF11 (NM_015885) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- PCF11 | PCF11p homolog | KIAA0824
- Origin Species
- Human
- NCBI GenBank
- NM_015885
- SwissProt
- O94913
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 173.5 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MSEQTPAEAG AAGAREDACR DYQSSLEDLT FNSKPHINML TILAEENLPF AKEIVSLIEA61QTAKAPSSEK LPVMYLMDSI VKNVGREYLT AFTKNLVATF ICVFEKVDEN TRKSLFKLRS121TWDEIFPLKK LYALDVRVNS LDPAWPIKPL PPNVNTSSIH VNPKFLNKSP EEPSTPGTVV181SSPSISTPPI VPDIQKNLTQ EQLIRQQLLA KQKQLLELQQ KKLELELEQA KAQLAVSLSV241QQETSNLGPG SAPSKLHVSQ IPPMAVKAPH QVPVQSEKSR PGPSLQIQDL KGTNRDPRLN301RISQHSHGKD QSHRKEFLMN TLNQSDTKTS KTIPSEKLNS SKQEKSKSGE KITKKELDQL361DSKSKSKSKS PSPLKNKLSH TKDLKNQESE SMRLSDMNKR DPRLKKHLQD KTDGKDDDVK421EKRKTAEKKD KDEHMKSSEH RLAGSRNKII NGIVQKQDTI TEESEKQGTK PGRSSTRKRS481RSRSPKSRSP IIHSPKRRDR RSPKRRQRSM SPTSTPKAGK IRQSGAKQSH MEEFTPPSRE541DRNAKRSTKQ DIRDPRRMKK TEEERPQETT NQHSTKSGTE PKENVENWQS SKSAKRWKSG601WEENKSLQQV DEHSKPPHLR HRESWSSTKG ILSPRAPKQQ QHRLSVDANL QIPKELTLAS661KRELLQKTSE RLASGEITQD DFLVVVHQIR QLFQYQEGVR EEQRSPFNDR FPLKRPRYED721SDKPFVDSPA SRFAGLDTNQ RLTALAEDRP LFDGPSRPSV ARDGPTKMIF EGPNKLSPRI781DGPPTPASLR FDGSPGQMGG GGPLRFEGPQ GQLGGGCPLR FEGPPGPVGT PLRFEGPIGQ841AGGGGFRFEG SPGLRFEGSP GGLRFEGPGG QPVGGLRFEG HRGQPVGGLR FEGPHGQPVG901GLRFDNPRGQ PVGGLRFEGG HGPSGAAIRF DGPHGQPGGG IRFEGPLLQQ GVGMRFEGPH961GQSVAGLRFE GQHNQLGGNL RFEGPHGQPG VGIRFEGPLV QQGGGMRFEG PSVPGGGLRI1021EGPLGQGGPR FEGCHALRFD GQPGQPSLLP RFDGLHGQPG PRFERTPGQP GPQRFDGPPG1081QQVQPRFDGV PQRFDGPQHQ QASRFDIPLG LQGTRFDNHP SQRLESVSFN QTGPYNDPPG1141NAFNAPSQGL QFQRHEQIFD SPQGPNFNGP HGPGNQSFSN PLNRASGHYF DEKNLQSSQF1201GNFGNIPAPM TVGNIQASQQ VLSGVAQPVA FGQGQQFLPV HPQNPGFVQN PSGALPKAYP1261DNHLSQVDVN ELFSKLLKTG ILKLSQTDSA TTQVSEVTAQ PPPEEEEDQN EDQDVPDLTN1321FTVEELKQRY DSVINRLYTG IQCYSCGMRF TTSQTDVYAD HLDWHYRQNR TEKDVSRKVT1381HRRWYYSLTD WIEFEEIADL EERAKSQFFE KVHEEVVLKT QEAAKEKEFQ SVPAGPAGAV1441ESCEICQEQF EQYWDEEEEE WHLKNAIRVD GKIYHPSCYE DYQNTSSFDC TPSPSKTPVE1501NPLNIMLNIV KNELQEPCDS PKVKEERIDT PPACTEESIA TPSEIKTEND TVESVTRTRPLEQKLISEEDLAANDILDYKDDDDKV
1555 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
