Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
PCDHB4 / Protocadherin Beta 4 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G101597-100
Catalog Number: LS-G101597
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PCDHB4 (NM_018938), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- PCDH-BETA4 | Protocadherin beta 4 | PCDH-beta-4 | PCDHB4 | Protocadherin beta-4
- Origin Species
- Human
- NCBI GenBank
- NM_018938
- SwissProt
- Q9Y5E5
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 84 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MKKLGRIHPN RQVLAFILMV FLSQVRLEPI RYSVLEETES GSFVAHLAKD LGLGIGELAS61RSARVLSDDD KQRLQLDRQT GDLLLREKLD REELCGPIEP CVLHFQVFLE MPVQFFQGEL121LIQDINDHSP IFPEREVLLK ILENSQPGTL FPLLIAEDLD VGSNGLQKYT ISPNSHFHIL181TRNHSEGKKY PDLVQDKPLD REEQPEFSLT LVALDGGSPP RSGTVMVRIL IMDINDNAPE241FVHTPYGVQV LENSPLDSPI VRVLARDIDA GNFGSVSYGL FQASDEIKQT FSINEVTGEI301LLKKKLDFEK IKSYHVEIEA TDGGGLSGKG TVVIEVVDVN DNPPELIISS LTSSIPENAP361ETVVSIFRIR DRDSGENGKM ICSIPDNLPF ILKPTLKNFY TLVTERPLDR ETSAEYNITI421AVTDLGTPRL KTQQNITVQV SDVNDNAPAF TQTSYTLFVR ENNSPALHIG SVSATDRDSG481TNAQVTYSLL PPQDPHLPLA SLVSINADNG HLFALRSLDY EALQAFEFRV GASDRGSPAL541SSEALVRVLV LDTNDNSPFV LYPLQNGSAP CTELVPRAAE PGYLVTKVVA VDGDSGQNAW601LSYQLLKATE PGLFGVWAHN GEVRTARLLS ERDAAKHRLV VLVKDNGEPP RSATATLHVL661LVDGFSQPYL PLPEAAPAQA QADSLTVYLV VALASVSSLF LFSVLLFVAV RLCRRSRAAS721VGRCSVPEGP FPGHLVDVSG TGTLSQSYQY EVCLTGDSGT GEFKFLKPIF PNLLVQDTGR781EVKENPKFRN SLVFSTRTRPLEQKLISEEDLAANDILDYKDDDDKV
795 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.