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Human PCDHB4 / Protocadherin Beta 4 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human PCDHB4 / Protocadherin Beta 4 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
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$220.00each
Catalog Number: LS-G91082-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human PCDHB4 (NM_018938) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
PCDH-BETA4 | Protocadherin beta 4 | PCDH-beta-4 | PCDHB4 | Protocadherin beta-4
Origin Species
Human
NCBI GenBank
NM_018938
SwissProt
Q9Y5E5

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
84 kDa
Protein Type
Lysate
Amino Acid Sequence
1MKKLGRIHPN RQVLAFILMV FLSQVRLEPI RYSVLEETES GSFVAHLAKD LGLGIGELAS
61RSARVLSDDD KQRLQLDRQT GDLLLREKLD REELCGPIEP CVLHFQVFLE MPVQFFQGEL
121LIQDINDHSP IFPEREVLLK ILENSQPGTL FPLLIAEDLD VGSNGLQKYT ISPNSHFHIL
181TRNHSEGKKY PDLVQDKPLD REEQPEFSLT LVALDGGSPP RSGTVMVRIL IMDINDNAPE
241FVHTPYGVQV LENSPLDSPI VRVLARDIDA GNFGSVSYGL FQASDEIKQT FSINEVTGEI
301LLKKKLDFEK IKSYHVEIEA TDGGGLSGKG TVVIEVVDVN DNPPELIISS LTSSIPENAP
361ETVVSIFRIR DRDSGENGKM ICSIPDNLPF ILKPTLKNFY TLVTERPLDR ETSAEYNITI
421AVTDLGTPRL KTQQNITVQV SDVNDNAPAF TQTSYTLFVR ENNSPALHIG SVSATDRDSG
481TNAQVTYSLL PPQDPHLPLA SLVSINADNG HLFALRSLDY EALQAFEFRV GASDRGSPAL
541SSEALVRVLV LDTNDNSPFV LYPLQNGSAP CTELVPRAAE PGYLVTKVVA VDGDSGQNAW
601LSYQLLKATE PGLFGVWAHN GEVRTARLLS ERDAAKHRLV VLVKDNGEPP RSATATLHVL
661LVDGFSQPYL PLPEAAPAQA QADSLTVYLV VALASVSSLF LFSVLLFVAV RLCRRSRAAS
721VGRCSVPEGP FPGHLVDVSG TGTLSQSYQY EVCLTGDSGT GEFKFLKPIF PNLLVQDTGR
781EVKENPKFRN SLVFS
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

795 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.