PAX7 Antibody (clone PAX7/497)
Catalog Number: LS-C358012
2 sizes / from $379.00
Antibody: Mouse Monoclonal
Applications: Flow Cytometry, Western Blot
Reactivity: Human, Mouse, Rat
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human PAX7 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$220.00each
Catalog Number: LS-G77905-20
Catalog Number: LS-G77905
For bulk orders or custom formulations:
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human PAX7 (NM_013945) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- HUP1 | Paired box 7 | Paired box gene 7 | PAX7 | Paired box homeotic gene 7 | Paired box protein Pax-7 | RMS2 | Paired domain gene 7 | PAX7 transcriptional factor | PAX7B
- Origin Species
- Human
- NCBI GenBank
- NM_002584
- SwissProt
- P23759
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 56.5 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MAALPGTVPR MMRPAPGQNY PRTGFPLEVS TPLGQGRVNQ LGGVFINGRP LPNHIRHKIV61EMAHHGIRPC VISRQLRVSH GCVSKILCRY QETGSIRPGA IGGSKPRQVA TPDVEKKIEE121YKRENPGMFS WEIRDRLLKD GHCDRSTVPS VSSISRVLRI KFGKKEEEDE ADKKEDDGEK181KAKHSIDGIL GDKGNRLDEG SDVESEPDLP LKRKQRRSRT TFTAEQLEEL EKAFERTHYP241DIYTREELAQ RTKLTEARVQ VWFSNRRARW RKQAGANQLA AFNHLLPGGF PPTGMPTLPP301YQLPDSTYPT TTISQDGGST VHRPQPLPPS TMHQGGLAAA AAAADTSSAY GARHSFSSYS361DSFMNPAAPS NHMNPVSNGL SPQVMSILGN PSAVPPQPQA DFSISPLHGG LDSATSISAS421CSQRADSIKP GDSLPTSQAY CPPTYSTTGY SVDPVAGYQY GQYGQSECLV PWASPVPIPS481PTPRASCLFM ESYKVVSGWG MSISQMEKLK SSQMEQFTTRTRPLEQKLISEEDLAANDILDYKDDDDKV
518 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
