LSBio
PAX3 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

PAX3 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all PAX3 antibodies →
$725.00each
Catalog Number: LS-G104515-100

For bulk orders or custom formulations:

Request a Quote

Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PAX3 (NM_181458), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
CDHS | Paired domain gene 3 | Paired box protein Pax-3 | Paired domain gene HuP2 | PAX3 | Waardenburg syndrome 1 | WS1 | HUP2 | Paired box 3 | Paired box homeotic gene 3 | WS3
Origin Species
Human
NCBI GenBank
NM_000438

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
53.3 kDa
Protein Type
Lysate
Amino Acid Sequence
1MTTLAGAVPR MMRPGPGQNY PRSGFPLEVS TPLGQGRVNQ LGGVFINGRP LPNHIRHKIV
61EMAHHGIRPC VISRQLRVSH GCVSKILCRY QETGSIRPGA IGGSKPKVTT PDVEKKIEEY
121KRENPGMFSW EIRDKLLKDA VCDRNTVPSV SSISRILRSK FGKGEEEEAD LERKEAEESE
181KKAKHSIDGI LSERASAPQS DEGSDIDSEP DLPLKRKQRR SRTTFTAEQL EELERAFERT
241HYPDIYTREE LAQRAKLTEA RVQVWFSNRR ARWRKQAGAN QLMAFNHLIP GGFPPTAMPT
301LPTYQLSETS YQPTSIPQAV SDPSSTVHRP QPLPPSTVHQ STIPSNPDSS SAYCLPSTRH
361GFSSYTDSFV PPSGPSNPMN PTIGNGLSPQ VMGLLTNHGG VPHQPQTDYA LSPLTGGLEP
421TTTVSASCSQ RLDHMKSLDS LPTSQSYCPP TYSTTGYSMD PVTGYQYGQY GQSAFHYLKP
481DIA
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

483 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.