PAX3 Antibody
Catalog Number: LS-C192855
2 sizes / from $364.00
Antibody: Rabbit Polyclonal
Applications: Immunohistochemistry (general), Western Blot
Reactivity: Human, Mouse, Rat
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human PAX3 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$220.00each
Catalog Number: LS-G91281-20
Catalog Number: LS-G91281
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human PAX3 (NM_181458) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- CDHS | Paired domain gene 3 | Paired box protein Pax-3 | Paired domain gene HuP2 | PAX3 | Waardenburg syndrome 1 | WS1 | HUP2 | Paired box 3 | Paired box homeotic gene 3 | WS3
- Origin Species
- Human
- NCBI GenBank
- NM_000438
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 53.3 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MTTLAGAVPR MMRPGPGQNY PRSGFPLEVS TPLGQGRVNQ LGGVFINGRP LPNHIRHKIV61EMAHHGIRPC VISRQLRVSH GCVSKILCRY QETGSIRPGA IGGSKPKVTT PDVEKKIEEY121KRENPGMFSW EIRDKLLKDA VCDRNTVPSV SSISRILRSK FGKGEEEEAD LERKEAEESE181KKAKHSIDGI LSERASAPQS DEGSDIDSEP DLPLKRKQRR SRTTFTAEQL EELERAFERT241HYPDIYTREE LAQRAKLTEA RVQVWFSNRR ARWRKQAGAN QLMAFNHLIP GGFPPTAMPT301LPTYQLSETS YQPTSIPQAV SDPSSTVHRP QPLPPSTVHQ STIPSNPDSS SAYCLPSTRH361GFSSYTDSFV PPSGPSNPMN PTIGNGLSPQ VMGLLTNHGG VPHQPQTDYA LSPLTGGLEP421TTTVSASCSQ RLDHMKSLDS LPTSQSYCPP TYSTTGYSMD PVTGYQYGQY GQSAFHYLKP481DIATRTRPLEQKLISEEDLAANDILDYKDDDDKV
483 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
