Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
PARPBP / C12orf48 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G112119-100
Catalog Number: LS-G112119
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human C12orf48(PARPBP) (NM_017915), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- AROM | C12orf48 | PARP1-binding protein | PARPBP | PARI | PARP-1 binding protein | PARP1 binding protein | PCNA-interacting partner
- Origin Species
- Human
- NCBI GenBank
- NM_017915
- SwissProt
- Q9NWS1
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 65.05 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MAVFNQKSVS DMIKEFRKNW RALCNSERTT LCGADSMLLA LQLSMAENNK QHSGEFTVSL61SDVLLTWKYL LHEKLNLPVE NMDVTDHYED VRKIYDDFLK NSNMLDLIDV YQKCRALTSN121CENYNTVSPS QLLDFLSGKQ YAVGDETDLS IPTSPTSKYN RDNEKVQLLA RKIIFSYLNL181LVNSKNDLAV AYILNIPDRG LGREAFTDLK HAAREKQMSI FLVATSFIRT IELGGKGYAP241PPSDPLRTHV KGLSNFINFI DKLDEILGEI PNPSIAGGQI LSVIKMQLIK GQNSRDPFCK301AIEEVAQDLD LRIKNIINSQ EGVVALSTTD ISPARPKSHA INHGTAYCGR DTVKALLVLL361DEEAANAPTK NKAELLYDEE NTIHHHGTSI LTLFRSPTQV NNSIKPLRER ICVSMQEKKI421KMKQTLIRSQ FACTYKDDYM ISKDNWNNVN LASKPLCVLY MENDLSEGVN PSVGRSTIGT481SFGNVHLDRS KNEKVSRKST SQTGNKSSKR KQVDLDGENI LCDNRNEPPQ HKNAKIPKKS541NDSQNRLYGK LAKVAKSNKC TAKDKLISGQ AKLTQFFRLTRTRPLEQKLISEEDLAANDILDYKDDDDKV
579 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.