LSBio
Human PARPBP / C12orf48 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human PARPBP / C12orf48 Protein (Over-Expression Lysate Myc-DDK (Flag))

Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all PARPBP antibodies →
$220.00each
Catalog Number: LS-G86765-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human C12orf48(PARPBP) (NM_017915) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
AROM | C12orf48 | PARP1-binding protein | PARPBP | PARI | PARP-1 binding protein | PARP1 binding protein | PCNA-interacting partner
Origin Species
Human
NCBI GenBank
NM_017915
SwissProt
Q9NWS1

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
65.05 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAVFNQKSVS DMIKEFRKNW RALCNSERTT LCGADSMLLA LQLSMAENNK QHSGEFTVSL
61SDVLLTWKYL LHEKLNLPVE NMDVTDHYED VRKIYDDFLK NSNMLDLIDV YQKCRALTSN
121CENYNTVSPS QLLDFLSGKQ YAVGDETDLS IPTSPTSKYN RDNEKVQLLA RKIIFSYLNL
181LVNSKNDLAV AYILNIPDRG LGREAFTDLK HAAREKQMSI FLVATSFIRT IELGGKGYAP
241PPSDPLRTHV KGLSNFINFI DKLDEILGEI PNPSIAGGQI LSVIKMQLIK GQNSRDPFCK
301AIEEVAQDLD LRIKNIINSQ EGVVALSTTD ISPARPKSHA INHGTAYCGR DTVKALLVLL
361DEEAANAPTK NKAELLYDEE NTIHHHGTSI LTLFRSPTQV NNSIKPLRER ICVSMQEKKI
421KMKQTLIRSQ FACTYKDDYM ISKDNWNNVN LASKPLCVLY MENDLSEGVN PSVGRSTIGT
481SFGNVHLDRS KNEKVSRKST SQTGNKSSKR KQVDLDGENI LCDNRNEPPQ HKNAKIPKKS
541NDSQNRLYGK LAKVAKSNKC TAKDKLISGQ AKLTQFFRL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

579 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.