Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
PARD6A / PAR6 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G120493-100
Catalog Number: LS-G120493
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PAR6(PARD6A) (NM_001037281), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- PAR-6 alpha | PARD6A | PAR6A | Tax interaction protein 40 | Tax-interacting protein 40 | PAR-6 | PAR-6A | PAR6C | TIP-40 | PAR6 | PAR6alpha | TAX40
- Origin Species
- Human
- NCBI GenBank
- NM_016948
- SwissProt
- Q9NPB6
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 37.1 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MARPQRTPAR SPDSIVEVKS KFDAEFRRFA LPRASVSGFQ EFSRLLRAVH QIPGLDVLLG61YTDAHGDLLP LTNDDSLHRA LASGPPPLRL LVQKREADSS GLAFASNSLQ RRKKGLLLRP121VAPLRTRPPL LISLPQDFRQ VSSVIDVDLL PETHRRVRLH KHGSDRPLGF YIRDGMSVRV181APQGLERVPG IFISRLVRGG LAESTGLLAV SDEILEVNGI EVAGKTLDQV TDMMVANSHN241LIVTVKPANQ RNNVVRGASG RLTGPPSAGP GPAEPDSDDD SSDLVIENRQ PPSSNGLSQG301PPCWDLHPGC RHPGTRSSLP SLDDQEQASS GWGSRIRGDG SGFSLTRTRPLEQKLISEEDLAANDILDYKDDDDKV
345 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.