Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
PAICS / ADE2 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G120086-100
Catalog Number: LS-G120086
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PAICS (NM_001079525), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- ADE2 | ADE2H1 | AIR carboxylase | AIRC | Multifunctional protein ADE2H1 | PAIS | Multifunctional protein ADE2 | SAICAR synthetase | PAICS
- Origin Species
- Human
- NCBI GenBank
- NM_006452
- SwissProt
- P22234
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 47.8 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MATAEVLNIG KKLYEGKTKE VYELLDSPGK VLLQSKDQIT AGNAARKNHL EGKAAISNKI61TSCIFQLLQE AVTSYKSNRI KTAFTRKCGE TAFIAPQCEM IPIEWVCRRI ATGSFLKRNP121GVKEGYKFYP PKVELFFKDD ANNDPQWSEE QLIAAKFCFA GLLIGQTEVD IMSHATQAIF181EILEKSWLPQ NCTLVDMKIE FGVDVTTKEI VLADVIDNDS WRLWPSGDRS QQKDKQSYRD241LKEVTPEGLQ MVKKNFEWVA ERVELLLKSE SQCRVVVLMG STSDLGHCEK IKKACGNFGI301PCELRVTSAH KGPDETLRIK AEYEGDGIPT VFVAVAGRSN GLGPVMSGNT AYPVISCPPL361TPDWGVQDVW SSLRLPSGLG CSTVLSPEGS AQFAAQIFGL SNHLVWSKLR ASILNTWISL421KQADKKIREC NLTRTRPLEQKLISEEDLAANDILDYKDDDDKV
432 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.