PAICS / ADE2 Antibody
Catalog Number: LS-C334729
2 sizes / from $281.00
Antibody: Rabbit Polyclonal
Applications: Immunofluorescence, Immunohistochemistry (general), Western Blot
Reactivity: Human, Mouse, Rat
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human PAICS / ADE2 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$220.00each
Catalog Number: LS-G77461-20
Catalog Number: LS-G77461
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human PAICS (NM_001079525) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- ADE2 | ADE2H1 | AIR carboxylase | AIRC | Multifunctional protein ADE2H1 | PAIS | Multifunctional protein ADE2 | SAICAR synthetase | PAICS
- Origin Species
- Human
- NCBI GenBank
- NM_006452
- SwissProt
- P22234
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 47.8 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MATAEVLNIG KKLYEGKTKE VYELLDSPGK VLLQSKDQIT AGNAARKNHL EGKAAISNKI61TSCIFQLLQE AVTSYKSNRI KTAFTRKCGE TAFIAPQCEM IPIEWVCRRI ATGSFLKRNP121GVKEGYKFYP PKVELFFKDD ANNDPQWSEE QLIAAKFCFA GLLIGQTEVD IMSHATQAIF181EILEKSWLPQ NCTLVDMKIE FGVDVTTKEI VLADVIDNDS WRLWPSGDRS QQKDKQSYRD241LKEVTPEGLQ MVKKNFEWVA ERVELLLKSE SQCRVVVLMG STSDLGHCEK IKKACGNFGI301PCELRVTSAH KGPDETLRIK AEYEGDGIPT VFVAVAGRSN GLGPVMSGNT AYPVISCPPL361TPDWGVQDVW SSLRLPSGLG CSTVLSPEGS AQFAAQIFGL SNHLVWSKLR ASILNTWISL421KQADKKIREC NLTRTRPLEQKLISEEDLAANDILDYKDDDDKV
432 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
