LSBio
Human PAICS / ADE2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human PAICS / ADE2 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
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$220.00each
Catalog Number: LS-G77461-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human PAICS (NM_001079525) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
ADE2 | ADE2H1 | AIR carboxylase | AIRC | Multifunctional protein ADE2H1 | PAIS | Multifunctional protein ADE2 | SAICAR synthetase | PAICS
Origin Species
Human
NCBI GenBank
NM_006452
SwissProt
P22234

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
47.8 kda
Protein Type
Lysate
Amino Acid Sequence
1MATAEVLNIG KKLYEGKTKE VYELLDSPGK VLLQSKDQIT AGNAARKNHL EGKAAISNKI
61TSCIFQLLQE AVTSYKSNRI KTAFTRKCGE TAFIAPQCEM IPIEWVCRRI ATGSFLKRNP
121GVKEGYKFYP PKVELFFKDD ANNDPQWSEE QLIAAKFCFA GLLIGQTEVD IMSHATQAIF
181EILEKSWLPQ NCTLVDMKIE FGVDVTTKEI VLADVIDNDS WRLWPSGDRS QQKDKQSYRD
241LKEVTPEGLQ MVKKNFEWVA ERVELLLKSE SQCRVVVLMG STSDLGHCEK IKKACGNFGI
301PCELRVTSAH KGPDETLRIK AEYEGDGIPT VFVAVAGRSN GLGPVMSGNT AYPVISCPPL
361TPDWGVQDVW SSLRLPSGLG CSTVLSPEGS AQFAAQIFGL SNHLVWSKLR ASILNTWISL
421KQADKKIREC NL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

432 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.