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PADI1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

PADI1 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all PADI1 antibodies →
$725.00each
Catalog Number: LS-G114326-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PADI1(PADI1) (NM_013358), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
HPAD-colony 10 | PAD1 | PADI1 | HPAD10 | PDI | Peptidylarginine deiminase I
Origin Species
Human
NCBI GenBank
NM_013358
SwissProt
Q9ULC6

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
74.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAPKRVVQLS LKMPTHAVCV VGVEAHVDIH SDVPKGANSF RVSGSSGVEV FMVYNRTRVK
61EPIGKARWPL DTDADMVVSV GTASKELKDF KVRVSYFGEQ EDQALGRSVL YLTGVDISLE
121VDTGRTGKVK RSQGDKKTWR WGPEGYGAIL LVNCDRDNHR SAEPDLTHSW LMSLADLQDM
181SPMLLSCNGP DKLFDSHKLV LNVPFSDSKR VRVFCARGGN SLSDYKQVLG PQCLSYEVER
241QPGEQEIKFY VEGLTFPDAD FLGLVSLSVS LVDPGTLPEV TLFTDTVGFR MAPWIMTPNT
301QPPEELYVCR VMDTHGSNEK FLEDMSYLTL KANCKLTICP QVENRNDRWI QDEMEFGYIE
361APHKSFPVVF DSPRNRGLKD FPYKRILGPD FGYVTREIPL PGPSSLDSFG NLDVSPPVTV
421GGTEYPLGRI LIGSSFPKSG GRQMARAVRN FLKAQQVQAP VELYSDWLSV GHVDEFLTFV
481PTSDQKGFRL LLASPSACLK LFQEKKEEGY GEAAQFDGLK HQAKRSINEM LADRHLQRDN
541LHAQKCIDWN RNVLKRELGL AESDIVDIPQ LFFLKNFYAE AFFPDMVNMV VLGKYLGIPK
601PYGPIINGRC CLEEKVQSLL EPLGLHCIFI DDYLSYHELQ GEIHCGTNVR RKPFPFKWWN
661MVP
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

663 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.