PADI1 Antibody
Catalog Number: LS-C153532
2 sizes / from $449.00
Antibody: Rabbit Polyclonal
Applications: Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Western Blot
Reactivity: Human
Format: Lyophilized
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human PADI1 Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$220.00each
Catalog Number: LS-G91373-20
Catalog Number: LS-G91373
For bulk orders or custom formulations:
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human PADI1(PADI1) (NM_013358) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- HPAD-colony 10 | PAD1 | PADI1 | HPAD10 | PDI | Peptidylarginine deiminase I
- Origin Species
- Human
- NCBI GenBank
- NM_013358
- SwissProt
- Q9ULC6
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 74.5 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MAPKRVVQLS LKMPTHAVCV VGVEAHVDIH SDVPKGANSF RVSGSSGVEV FMVYNRTRVK61EPIGKARWPL DTDADMVVSV GTASKELKDF KVRVSYFGEQ EDQALGRSVL YLTGVDISLE121VDTGRTGKVK RSQGDKKTWR WGPEGYGAIL LVNCDRDNHR SAEPDLTHSW LMSLADLQDM181SPMLLSCNGP DKLFDSHKLV LNVPFSDSKR VRVFCARGGN SLSDYKQVLG PQCLSYEVER241QPGEQEIKFY VEGLTFPDAD FLGLVSLSVS LVDPGTLPEV TLFTDTVGFR MAPWIMTPNT301QPPEELYVCR VMDTHGSNEK FLEDMSYLTL KANCKLTICP QVENRNDRWI QDEMEFGYIE361APHKSFPVVF DSPRNRGLKD FPYKRILGPD FGYVTREIPL PGPSSLDSFG NLDVSPPVTV421GGTEYPLGRI LIGSSFPKSG GRQMARAVRN FLKAQQVQAP VELYSDWLSV GHVDEFLTFV481PTSDQKGFRL LLASPSACLK LFQEKKEEGY GEAAQFDGLK HQAKRSINEM LADRHLQRDN541LHAQKCIDWN RNVLKRELGL AESDIVDIPQ LFFLKNFYAE AFFPDMVNMV VLGKYLGIPK601PYGPIINGRC CLEEKVQSLL EPLGLHCIFI DDYLSYHELQ GEIHCGTNVR RKPFPFKWWN661MVPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
663 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
