Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
P2Y13 / P2RY13 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G104888-100
Catalog Number: LS-G104888
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human GPR86(P2RY13) (NM_176894), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- FKSG77 | G-protein coupled receptor 94 | GPR94 | G-protein coupled receptor 86 | GPCR1 | GPR86 | p2RY13 | SP174 | G protein-coupled receptor 86 | p2Y purinoceptor 13 | p2Y13
- Origin Species
- Human
- NCBI GenBank
- NM_023914
- SwissProt
- Q9BPV8
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 38.2 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MNTTVMQGFN RSERCPRDTR IVQLVFPALY TVVFLTGILL NTLALWVFVH IPSSSTFIIY61LKNTLVADLI MTLMLPFKIL SDSHLAPWQL RAFVCRFSSV IFYETMYVGI VLLGLIAFDR121FLKIIRPLRN IFLKKPVFAK TVSIFIWFFL FFISLPNMIL SNKEATPSSV KKCASLKGPL181GLKWHQMVNN ICQFIFWTVF ILMLVFYVVI AKKVYDSYRK SKSKDRKNNK KLEGKVFVVV241AVFFVCFAPF HFARVPYTHS QTNNKTDCRL QNQLFIAKET TLFLAATNIC MDPLIYIFLC301KKFTEKLPCM QGRKTTASSQ ENHSSQTDNI TLGTRTRPLEQKLISEEDLAANDILDYKDDDDKV
333 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.