LSBio
Human P2Y13 / P2RY13 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human P2Y13 / P2RY13 Protein (Over-Expression Lysate Myc-DDK (Flag))

Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all P2RY13 antibodies →
$153.00each
Catalog Number: LS-G88142-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human GPR86(P2RY13) (NM_023914) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
FKSG77 | G-protein coupled receptor 94 | GPR94 | G-protein coupled receptor 86 | GPCR1 | GPR86 | p2RY13 | SP174 | G protein-coupled receptor 86 | p2Y purinoceptor 13 | p2Y13
Origin Species
Human
NCBI GenBank
NM_023914
SwissProt
Q9BPV8

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
38.9 kda
Protein Type
Lysate
Amino Acid Sequence
1MNTTVMQGFN RSERCPRDTR IVQLVFPALY TVVFLTGILL NTLALWVFVH IPSSSTFIIY
61LKNTLVADLI MTLMLPFKIL SDSHLAPWQL RAFVCRFSSV IFYETMYVGI VLLGLIAFDR
121FLKIIRPLRN IFLKKPVFAK TVSIFIWFFL FFISLPNMIL SNKEATPSSV KKCASLKGPL
181GLKWHQMVNN ICQFIFWTVF ILMLVFYVVI AKKVYDSYRK SKSKDRKNNK KLEGKVFVVV
241AVFFVCFAPF HFARVPYTHS QTNNKTDCRL QNQLFIAKET TLFLAATNIC MDPLIYIFLC
301KKFTEKLPCM QGRKTTASSQ ENHSSQTDNI TLG
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

333 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.