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OTUD4 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

OTUD4 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all OTUD4 antibodies →
$725.00each
Catalog Number: LS-G118830-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human OTUD4 (NM_001102653), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
DUBA6 | HIN1 | HIV-1 induced protein HIN-1 | OTUD4 | HIV-1-induced protein HIN-1 | HSHIN1 | KIAA1046 | OTU domain containing 4
Origin Species
Human
NCBI GenBank
NM_017493
SwissProt
Q01804

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
116.9 kDa
Protein Type
Lysate
Amino Acid Sequence
1MACIHYLREN REKFEAFIEG SFEEYLKRLE NPQEWVGQVE ISALSLMYRK DFIIYREPNV
61SPSQVTENNF PEKVLLCFSN GNHYDIVYPI KYKESSAMCQ SLLYELLYEK VFKTDVSKIV
121MELDTLEVAD EDNSEISDSE DDSCKSKTAA AAADVNGFKP LSGNEQLKNN GNSTSLPLSR
181KVLKSLNPAV YRNVEYEIWL KSKQAQQKRD YSIAAGLQYE VGDKCQVRLD HNGKFLNADV
241QGIHSENGPV LVEELGKKHT SKNLKAPPPE SWNTVSGKKM KKPSTSGQNF HSDVDYRGPK
301NPSKPIKAPS ALPPRLQHPS GVRQHAFSSH SSGSQSQKFS SEHKNLSRTP SQIIRKPDRE
361RVEDFDHTSR ESNYFGLSPE ERREKQAIEE SRLLYEIQNR DEQAFPALSS SSVNQSASQS
421SNPCVQRKSS HVGDRKGSRR RMDTEERKDK DSIHGHSQLD KRPEPSTLEN ITDDKYATVS
481SPSKSKKLEC PSPAEQKPAE HVSLSNPAPL LVSPEVHLTP AVPSLPATVP AWPSEPTTFG
541PTGVPAPIPV LSVTQTLTTG PDSAVSQAHL TPSPVPVSIQ AVNQPLMPLP QTLSLYQDPL
601YPGFPCNEKG DRAIVPPYSL CQTGEDLPKD KNILRFFFNL GVKAYSCPMW APHSYLYPLH
661QAYLAACRMY PKVPVPVYPH NPWFQEAPAA QNESDCTCTD AHFPMQTEAS VNGQMPQPEI
721GPPTFSSPLV IPPSQVSESH GQLSYQADLE SETPGQLLHA DYEESLSGKN MFPQPSFGPN
781PFLGPVPIAP PFFPHVWYGY PFQGFIENPV MRQNIVLPSD EKGELDLSLE NLDLSKDCGS
841VSTVDEFPEA RGEHVHSLPE ASVSSKPDEG RTEQSSQTRK ADTALASIPP VAEGKAHPPT
901QILNRERETV PVELEPKRTI QSLKEKTEKV KDPKTAADVV SPGANSVDSR VQRPKEESSE
961DENEVSNILR SGRSKQFYNQ TYGSRKYKSD WGYSGRGGYQ HVRSEESWKG QPSRSRDEGY
1021QYHRNVRGRP FRGDRRRSGM GDGHRGQHT
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1049 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.