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OTUD4 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

OTUD4 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all OTUD4 antibodies →
$725.00each
Catalog Number: LS-G103389-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human OTUD4 (NM_199324), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
DUBA6 | HIN1 | HIV-1 induced protein HIN-1 | OTUD4 | HIV-1-induced protein HIN-1 | HSHIN1 | KIAA1046 | OTU domain containing 4
Origin Species
Human
NCBI GenBank
NM_017493
SwissProt
Q01804

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
116.8 kDa
Protein Type
Lysate
Amino Acid Sequence
1MACIHYLREN REKFEAFIEG SFEEYLKRLE NPQEWVGQVE ISALSLMYRK DFIIYREPNV
61SPSQVTENNF PEKVLLCFSN GNHYDIVYPI KYKESSAMCQ SLLYELLYEK VFKTDVSKIV
121MELDTLEVAD EDNSEISDSE DDSCKSKTAA AAADVNGFKP LSGNELKNNG NSTSLPLSRK
181VLKSLNPAVY RNVEYEIWLK SKQAQQKRDY SIAAGLQYEV GDKCQVRLDH NGKFLNADVQ
241GIHSENGPVL VEELGKKHTS KNLKAPPPES WNTVSGKKMK KPSTSGQNFH SDVDYRGPKN
301PSKPIKAPSA LPPRLQHPSG VRQHAFSSHS SGSQSQKFSS EHKNLSRTPS QIIRKPDRER
361VEDFDHTSRE SNYFGLSPEE RREKQAIEES RLLYEIQNRD EQAFPALSSS SVNQSASQSS
421NPCVQRKSSH VGDRKGSRRR MDTEERKDKD SIHGHSQLDK RPEPSTLENI TDDKYATVSS
481PSKSKKLECP SPAEQKPAEH VSLSNPAPLL VSPEVHLTPA VPSLPATVPA WPSEPTTFGP
541TGVPAPIPVL SVTQTLTTGP DSAVSQAHLT PSPVPVSIQA VNQPLMPLPQ TLSLYQDPLY
601PGFPCNEKGD RAIVPPYSLC QTGEDLPKDK NILRFFFNLG VKAYSCPMWA PHSYLYPLHQ
661AYLAACRMYP KVPVPVYPHN PWFQEAPAAQ NESDCTCTDA HFPMQTEASV NGQMPQPEIG
721PPTFSSPLVI PPSQVSESHG QLSYQADLES ETPGQLLHAD YEESLSGKNM FPQPSFGPNP
781FLGPVPIAPP FFPHVWYGYP FQGFIENPVM RQNIVLPSDE KGELDLSLEN LDLSKDCGSV
841STVDEFPEAR GEHVHSLPEA SVSSKPDEGR TEQSSQTRKA DTALASIPPV AEGKAHPPTQ
901ILNRERETVP VELEPKRTIQ SLKEKTEKVK DPKTAADVVS PGANSVDSRV QRPKEESSED
961ENEVSNILRS GRSKQFYNQT YGSRKYKSDW GYSGRGGYQH VRSEESWKGQ PSRSRDEGYQ
1021YHRNVRGRPF RGDRRRSGMG DGHRGQHT
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1048 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.