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OTOA Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

OTOA Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all OTOA antibodies →
$725.00each
Catalog Number: LS-G128412-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human OTOA (NM_001161683), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Cancer/testis antigen 108 | CT108 | DFNB22 | Otoancorin | OTOA
Origin Species
Human
NCBI GenBank
NM_001161683
SwissProt
Q7RTW8

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
117.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MGNSITYRDQ AAVENHLEQR LHQPQKLLED LRKTDAQQFR TAMKCLLEDK KDGLDLKDII
61IDLGEIRERA LQSPGVNRSL FLITLERCFQ MLNSLECVEI LGKVLRGSSG SFLQPDITER
121LPRDLREDAF KNLSAVFKDL YDKTSAHSQR ALYSWMTGIL QTSSNATDDS ASWVSAEHLW
181VLGRYMVHLS FEEITKISPI EIGLFISYDN ATKQLDMVYD ITPELAQAFL ERISSSNFNM
241RNTSTIHRLG LLVCFYNDLE LLDATVAQVL LYQMIKCSHL RGFQAGVQKL KAELLDIAME
301NQTLNETLGS LSDAVVGLTY SQLESLSPEA VHGAISTLNQ VSGWAKSQVI ILSAKYLAHE
361KVLSFYNVSQ MGALLAGVST QAFCSMKRKD ISQVLRSAVS QYVSDLSPAQ QQGILSKMVQ
421AEDTAPGIVE IQGAFFKEVS LFDLRRQPGF NSTVLKDKEL GRSQALFLYE LLLKTTRRPE
481ELLSAGQLVK GVTCSHIDAM STDFFLAHFQ DFQNNFALLS PYQVNCLAWK YWEVSRLSMP
541PFLLAALPAR YLASVPASQC VPFLISLGKS WLDSLVLDSH KKTSVLRKVQ QCLDDSIADE
601YTVDIMGNLL CHLPAAIIDR GISPRAWATA LHGLRDCPDL NPEQKAAVRL KLLGQYGLPQ
661HWTAETTKDL GPFLVLFSGD ELSSIATKFP EILLQAASKM ARTLPTKEFL WAVFQSVRNS
721SDKIPSYDPM PGCHGVVAPS SDDIFKLAEA NACWALEDLR CMEEDTFIRT VELLGAVQGF
781SRPQLMTLKE KAIQVWDMPS YWREHHIVSL GRIALALNES ELEQLDLSSI DTVASLSWQT
841EWTPGQAESI LQGYLDDSGY SIQDLKSFHL VGLGATLCAI NITEIPLIKI SEFRVVVARI
901GTLLCSTHVL AEFKRKAEVV FGDPTEWTSS VLQELGTIAA GLTKAELRML DKDLMPYFQP
961SAIKCLPDEI FKELSAEQIA SLGPENAAAV THAQRRRLSP LQLQSLQQAL DGAKTHSWQD
1021APASAGPTRT SSSRSPAGAL QSWGLWLGCP LLVLMAKLLW
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1060 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.