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OTOA Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

OTOA Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all OTOA antibodies →
$725.00each
Catalog Number: LS-G106924-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human OTOA (NM_144672), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Cancer/testis antigen 108 | CT108 | DFNB22 | Otoancorin | OTOA
Origin Species
Human
NCBI GenBank
NM_001161683
SwissProt
Q7RTW8

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
126.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MSQEPTTYSL FLFLFLSHGV SSYTVPNSRQ DLHPLLQNMA EEIIDGSYLN ALLDLIQFQS
61SHVWTDDLSH RVLAYLNSRN VAFTIPSLQA AVENHLEQRL HQPQKLLEDL RKTDAQQFRT
121AMKCLLEDKK DGLDLKDIII DLGEIRERAL QSPGVNRSLF LITLERCFQM LNSLECVEIL
181GKVLRGSSGS FLQPDITERL PRDLREDAFK NLSAVFKDLY DKTSAHSQRA LYSWMTGILQ
241TSSNATDDSA SWVSAEHLWV LGRYMVHLSF EEITKISPIE IGLFISYDNA TKQLDMVYDI
301TPELAQAFLE RISSSNFNMR NTSTIHRLGL LVCFYNDLEL LDATVAQVLL YQMIKCSHLR
361GFQAGVQKLK AELLDIAMEN QTLNETLGSL SDAVVGLTYS QLESLSPEAV HGAISTLNQV
421SGWAKSQVII LSAKYLAHEK VLSFYNVSQM GALLAGVSTQ AFCSMKRKDI SQVLRSAVSQ
481YVSDLSPAQQ QGILSKMVQA EDTAPGIVEI QGAFFKEVSL FDLRRQPGFN STVLKDKELG
541RSQALFLYEL LLKTTRRPEE LLSAGQLVKG VTCSHIDAMS TDFFLAHFQD FQNNFALLSP
601YQVNCLAWKY WEVSRLSMPP FLLAALPARY LASVPASQCV PFLISLGKSW LDSLVLDSHK
661KTSVLRKVQQ CLDDSIADEY TVDIMGNLLC HLPAAIIDRG ISPRAWATAL HGLRDCPDLN
721PEQKAAVRLK LLGQYGLPQH WTAETTKDLG PFLVLFSGDE LSSIATKFPE ILLQAASKMA
781RTLPTKEFLW AVFQSVRNSS DKIPSYDPMP GCHGVVAPSS DDIFKLAEAN ACWALEDLRC
841MEEDTFIRTV ELLGAVQGFS RPQLMTLKEK AIQVWDMPSY WREHHIVSLG RIALALNESE
901LEQLDLSSID TVASLSWQTE WTPGQAESIL QGYLDDSGYS IQDLKSFHLV GLGATLCAIN
961ITEIPLIKIS EFRVVVARIG TLLCSTHVLA EFKRKAEVVF GDPTEWTSSV LQELGTIAAG
1021LTKAELRMLD KDLMPYFQPS AIKCLPDEIF KELSAEQIAS LGPENAAAVT HAQRRRLSPL
1081QLQSLQQALD GAKTHSWQDA PASAGPTRTS SSRSPAGALQ SWGLWLGCPL LVLMAKLLW
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1139 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.