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OSBPL9 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

OSBPL9 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G106484-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human OSBPL9 (NM_148906), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
OSBPL9 | ORP-9 | ORP9 | OSBP-related protein 9 | OSBP4
Origin Species
Human
NCBI GenBank
NM_024586
SwissProt
Q96SU4

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
63.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MVESIKHCIV LLQIAKDQSN AEKHADGMIS TINPVDAIYQ PSPLEPVIST MPSQTVLPPE
61PVQLCKSEQR PSSLPVGPVL ATLGHHQTPT PNSTGSGHSP PSSSLTSPSH VNLSPNTVPE
121FSYSSSEDEF YDADEFHQSG SSPKRLIDSS GSASVLTHSS SGNSLKRPDT TESLNSSLSN
181GTSDADLFDS HDDRDDDAEA GSVEEHKSVI MHLLSQVRLG MDLTKVVLPT FILERRSLLE
241MYADFFAHPD LFVSISDQKD PKDRMVQVVK WYLSAFHAGR KGSVAKKPYN PILGEIFQCH
301WTLPNDTEEN TELVSEGPVP WVSKNSVTFV AEQVSHHPPI SAFYAECFNK KIQFNAHIWT
361KSKFLGMSIG VHNIGQGCVS CLDYDEHYIL TFPNGYGRSI LTVPWVELGG ECNINCSKTG
421YSANIIFHTK PFYGGKKHRI TAEIFSPNDK KSFCSIEGEW NGVMYAKYAT GENTVFVDTK
481KLPIIKKKVR KLEDQNEYES RSLWKDVTFN LKIRDIDAAT EAKHRLEERQ RAEARERKEK
541EIQWETRLFH EDGECWVYDE PLLKRLGAAK H
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

571 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.