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OSBPL9 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

OSBPL9 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all OSBPL9 antibodies →
$725.00each
Catalog Number: LS-G106483-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human OSBPL9 (NM_148907), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
OSBPL9 | ORP-9 | ORP9 | OSBP-related protein 9 | OSBP4
Origin Species
Human
NCBI GenBank
NM_024586
SwissProt
Q96SU4

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
70.1 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAFLLATCGG LDSGFVPSVQ DFDKKLTEAD AYLQILIEQL KLFDDKLQNC KEDEQRKKIE
61TLKETTNSMV ESIKHCIVLL QIAKSTINPV DAIYQPSPLE PVISTMPSQT VLPPEPVQLC
121KSEQRPSSLP VGPVLATLGH HQTPTPNSTG SGHSPPSSSL TSPSHVNLSP NTVPEFSYSS
181SEDEFYDADE FHQSGSSPKR LIDSSGSASV LTHSSSGNSL KRPDTTESLN SSLSNGTSDA
241DLFDSHDDRD DDAEAGSVEE HKSVIMHLLS QVRLGMDLTK VVLPTFILER RSLLEMYADF
301FAHPDLFVSI SDQKDPKDRM VQVVKWYLSA FHAGRKGSVA KKPYNPILGE IFQCHWTLPN
361DTEENTELVS EGPVPWVSKN SVTFVAEQVS HHPPISAFYA ECFNKKIQFN AHIWTKSKFL
421GMSIGVHNIG QGCVSCLDYD EHYILTFPNG YGRSILTVPW VELGGECNIN CSKTGYSANI
481IFHTKPFYGG KKHRITAEIF SPNDKKSFCS IEGEWNGVMY AKYATGENTV FVDTKKLPII
541KKKVRKLEDQ NEYESRSLWK DVTFNLKIRD IDAATEAKHR LEERQRAEAR ERKEKEIQWE
601TRLFHEDGEC WVYDEPLLKR LGAAKH
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

626 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.