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OSBPL3 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

OSBPL3 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G106682-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human OSBPL3 (NM_145322), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
KIAA0704 | OSBP-related protein 3 | OSBP3 | ORP-3 | ORP3 | Oxysterol-binding protein 3 | OSBPL3
Origin Species
Human
NCBI GenBank
NM_015550
SwissProt
Q9H4L5

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
93.6 kDa
Protein Type
Lysate
Amino Acid Sequence
1MMSDEKNLGV SQKLVSPSRS TSSCSSKQGS RQDSWEVVEG LRGEMNYTQE PPVQKGFLLK
61KRKWPLKGWH KRFFYLDKGI LKYAKSQTDI EREKLHGCID VGLSVMSVKK SSKCIDLDTE
121EHIYHLKVKS EEVFDEWVSK LRHHRMYRQN EIAMFPHEVN HFFSGSTITD SSSGVFDSIS
181SRKRSSISKQ NLFQTGSNVS FSCGGETRVP LWLQSSEDME KCSKDLAHCH AYLVEMSQLL
241QSMDVLHRTY SAPAINAIQV PKPFSGPVRL HSSNPNLSTL DFGEEKNYSD GSETSSEFSK
301MQEDLCHIAH KVYFTLRSAF NIMSAEREKL KQLMEQDASS SPSAQVIGLK NALSSENSRD
361ENRALVHQLS NESRLSITDS LSEFFDAQEV LLSPSSSENE ISDDDSYVSD ISDNLSLDNL
421SNDLDNERQT LGPVLDSGRE AKSRRRTCLP APCPSSSNIS LWNILRNNIG KDLSKVAMPV
481ELNEPLNTLQ RLCEELEYSE LLDKAAQIPS PLERMVYVAA FAISAYASSY YRAGSKPFNP
541VLGETYECIR EDKGFQFFSE QVSHHPPISA CHAESRNFVF WQDVRWKNKF WGKSMEIVPI
601GTTHVTLPVF GDHFEWNKVT SCIHNILSGQ RWIEHYGEIV IKNLHDDSCY CKVNFIKAKY
661WSTNAHEIEG TVFDRSGKAV HRLFGKWHES IYCGGGSSSA CVWRANPMPK GYEQYYSFTQ
721FALELNEMDP SSKSLLPPTD TRFRPDQRFL EEGNLEEAEI QKQRIEQLQR ERRRVLEENH
781VEHQPRFFRK SDDDSWVSNG TYLELRKDLG FSKLDHPVLW
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

820 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.