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OSBPL3 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

OSBPL3 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G106680-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human OSBPL3 (NM_145320), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
KIAA0704 | OSBP-related protein 3 | OSBP3 | ORP-3 | ORP3 | Oxysterol-binding protein 3 | OSBPL3
Origin Species
Human
NCBI GenBank
NM_015550
SwissProt
Q9H4L5

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
97.5 kda
Protein Type
Lysate
Amino Acid Sequence
1MSDEKNLGVS QKLVSPSRST SSCSSKQGSR QDSWEVVEGL RGEMNYTQEP PVQKGFLLKK
61RKWPLKGWHK RFFYLDKGIL KYAKSQTDIE REKLHGCIDV GLSVMSVKKS SKCIDLDTEE
121HIYHLKVKSE EVFDEWVSKL RHHRMYRQNE IAMFPHEVNH FFSGSTITDS SSGVFDSISS
181RKRSSISKQN LFQTGSNVSF SCGGETRVPL WLQSSEDMEK CSKDLAHCHA YLVEMSQLLQ
241SMDVLHRTYS APAINAIQVP KPFSGPVRLH SSNPNLSTLD FGEEKNYSDG SETSSEFSKM
301QEDLCHIAHK VYFTLRSAFN IMSAEREKLK QLMEQDASSS PSAQVIGLKN ALSSALAQNT
361DLKERLRRIH AESLLLDSPA VAKSGDNLAE ENSRDENRAL VHQLSNESRL SITDSLSEFF
421DAQEVLLSPS SSENEISDDD SYVSDISDNL SLDNLSNDLD NERQTLGPVL DSGREAKSRR
481RTCLPAPCPS SSNISLWNIL RNNIGKDLSK VAMPVELNEP LNTLQRLCEE LEYSELLDKA
541AQIPSPLERM VYVAAFAISA YASSYYRAGS KPFNPVLGET YECIREDKGF QFFSEQVSHH
601PPISACHAES RNFVFWQDVR WKNKFWGKSM EIVPIGTTHV TLPVFGDHFE WNKVTSCIHN
661ILSGQRWIEH YGEIVIKNLH DDSCYCKVNF IKAKYWSTNA HEIEGTVFDR SGKAVHRLFG
721KWHESIYCGG GSSSACVWRA NPMPKGYEQY YSFTQFALEL NEMDPSSKSL LPPTDTRFRP
781DQRFLEEGNL EEAEIQKQRI EQLQRERRRV LEENHVEHQP RFFRKSDDDS WVSNGTYLEL
841RKDLGFSKLD HPVLW
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

855 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.