Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
NXPE1 / FAM55A Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G106363-100
Catalog Number: LS-G106363
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human FAM55A(NXPE1) (NM_152315), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Origin Species
- Human
- NCBI GenBank
- NM_152315
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 46.7 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MSSPALTAGA SGKVMDFNNG TYLVSFTLFW EGQVSLSLLL IHPSEGASAL WRARNQGYDK61IIFKGKFVNG TSHVFTECGL TLNSNAELCE YLDDRDQEAF YCMKPQHMPC EALTYMTTRN121REVSYLTDKE NSLFHRSKVG VEMMKDRKHI DVTNCNKREK IEETCQVGMK PPVPGGYTLQ181GKWITTFCNQ VQLDTIKING CLKGKLIYLL GDSTLRQWIY YFPKVVKTLK FFDLHETGIF241KKHLLLDAER HTQIQWKKHS YPFVTFQLYS LIDHDYIPRE IDRLSGDKNT AIVITFGQHF301RPFPIDIFIR RAIGVQKAIE RLFLRSPATK VIIKTENIRE MHIETERFVD FHGYIHYLIM361KDIFKDLNVG IIDAWDMTIA YGTDTIHPPD HVIGNQINMF LNYICTRTRPLEQKLISEEDLAANDILDYKDDDDKV
405 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.