LSBio
Human NXPE1 / FAM55A Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human NXPE1 / FAM55A Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
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$153.00each
Catalog Number: LS-G95777-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human FAM55A(NXPE1) (NM_152315) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Origin Species
Human
NCBI GenBank
NM_152315

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
46.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MSSPALTAGA SGKVMDFNNG TYLVSFTLFW EGQVSLSLLL IHPSEGASAL WRARNQGYDK
61IIFKGKFVNG TSHVFTECGL TLNSNAELCE YLDDRDQEAF YCMKPQHMPC EALTYMTTRN
121REVSYLTDKE NSLFHRSKVG VEMMKDRKHI DVTNCNKREK IEETCQVGMK PPVPGGYTLQ
181GKWITTFCNQ VQLDTIKING CLKGKLIYLL GDSTLRQWIY YFPKVVKTLK FFDLHETGIF
241KKHLLLDAER HTQIQWKKHS YPFVTFQLYS LIDHDYIPRE IDRLSGDKNT AIVITFGQHF
301RPFPIDIFIR RAIGVQKAIE RLFLRSPATK VIIKTENIRE MHIETERFVD FHGYIHYLIM
361KDIFKDLNVG IIDAWDMTIA YGTDTIHPPD HVIGNQINMF LNYIC
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

405 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.