LSBio
NVL Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

NVL Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all NVL antibodies →
$725.00each
Catalog Number: LS-G117922-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human NVL (NM_002533), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Nuclear VCP-like | NVLp | NVL | Nuclear VCP-like protein
Origin Species
Human
NCBI GenBank
NM_002533
SwissProt
O15381

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
94.9 kDa
Protein Type
Lysate
Amino Acid Sequence
1MKPRPAGFVD NKLKQRVIQY LTSNKCGKYV DIGVLASDLQ RVYSIDYGRR KRNAFRIQVE
61KVFSIISSEK ELKNLTELED EHLAKRARQG EEDNEYTESY SDDDSSMEDY PDPQSANHMN
121SSLLSLYRKG NPDSVSNTPE MEQRETTSST PRISSKTGSI PLKTPAKDSE GGWFIDKTPS
181VKKDSFFLDL SCEKSNPKKP ITEIQDSKDS SLLESDMKRK GKLKNKGSKR KKEDLQEVDG
241EIEAVLQKKA KARGLEFQIS NVKFEDVGGN DMTLKEVCKM LIHMRHPEVY HHLGVVPPRG
301VLLHGPPGCG KTLLAHAIAG ELDLPILKVA APEIVSGVSG ESEQKLRELF EQAVSNAPCI
361IFIDEIDAIT PKREVASKDM ERRIVAQLLT CMDDLNNVAA TARVLVIGAT NRPDSLDPAL
421RRAGRFDREI CLGIPDEASR ERILQTLCRK LRLPQAFDFC HLAHLTPGFV GADLMALCRE
481AAMCAVNRVL MKLQEQQKKN PEMEDLPSKG VQEERLGTEP TSETQDELQR LLGLLRDQDP
541LSEEQMQGLC IELNDFIVAL SSVQPSAKRE GFVTVPNVTW ADIGALEDIR EELTMAILAP
601VRNPDQFKAL GLVTPAGVLL AGPPGCGKTL LAKAVANESG LNFISVKGPE LLNMYVGESE
661RAVRQVFQRA KNSAPCVIFF DEVDALCPRR SDRETGASVR VVNQLLTEMD GLEARQQVFI
721MAATNRPDII DPAILRPGRL DKTLFVGLPP PADRLAILKT ITKNGTKPPL DADVNLEAIA
781GDLRCDCYTG ADLSALVREA SICALRQEMA RQKSGNEKGE LKVSHKHFEE AFKKVRSSIS
841KKDQIMYERL QESLSR
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

856 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.