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NVL Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

NVL Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all NVL antibodies →
$725.00each
Catalog Number: LS-G103020-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human NVL (NM_206840), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Nuclear VCP-like | NVLp | NVL | Nuclear VCP-like protein
Origin Species
Human
NCBI GenBank
NM_002533
SwissProt
O15381

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
82.6 kda
Protein Type
Lysate
Amino Acid Sequence
1MEDYPDPQSA NHMNSSLLSL YRKGNPDSVS NTPEMEQRET TSSTPRISSK TGSIPLKTPA
61KDSEGGWFID KTPSVKKDSF FLDLSCEKSN PKKPITEIQD SKDSSLLESD MKRKGKLKNK
121GSKRKKEDLQ EVDGEIEAVL QKKAKARGLE FQISNVKFED VGGNDMTLKE VCKMLIHMRH
181PEVYHHLGVV PPRGVLLHGP PGCGKTLLAH AIAGELDLPI LKVAAPEIVS GVSGESEQKL
241RELFEQAVSN APCIIFIDEI DAITPKREVA SKDMERRIVA QLLTCMDDLN NVAATARVLV
301IGATNRPDSL DPALRRAGRF DREICLGIPD EASRERILQT LCRKLRLPQA FDFCHLAHLT
361PGFVGADLMA LCREAAMCAV NRVLMKLQEQ QKKNPEMEDL PSKGVQEERL GTEPTSETQD
421ELQRLLGLLR DQDPLSEEQM QGLCIELNDF IVALSSVQPS AKREGFVTVP NVTWADIGAL
481EDIREELTMA ILAPVRNPDQ FKALGLVTPA GVLLAGPPGC GKTLLAKAVA NESGLNFISV
541KGPELLNMYV GESERAVRQV FQRAKNSAPC VIFFDEVDAL CPRRSDRETG ASVRVVNQLL
601TEMDGLEARQ QVFIMAATNR PDIIDPAILR PGRLDKTLFV GLPPPADRLA ILKTITKNGT
661KPPLDADVNL EAIAGDLRCD CYTGADLSAL VREASICALR QEMARQKSGN EKGELKVSHK
721HFEEAFKKVR SSISKKDQIM YERLQESLSR
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

750 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.