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NRXN3 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

NRXN3 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G124119-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human NRXN3 (NM_001105250), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
C14orf60 | KIAA0743 | Neurexin III-beta | Neurexin 3 | Neurexin III | NRXN3 | Neurexin III-alpha | Neurexin-3-beta
Origin Species
Human
NCBI GenBank
NM_004796
SwissProt
Q9Y4C0

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
50.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MHLRIHARRS PPRRPAWTLG IWFLFWGCIV SSVWSSSNVA SSSSTSSSPG SHSQHEHHFH
61GSKHHSVPIS IYRSPVSLRG GHAGATYIFG KSGGLILYTW PANDRPSTRS DRLAVGFSTT
121VKDGILVRID SAPGLGDFLQ LHIEQGKIGV VFNIGTVDIS IKEERTPVND GKYHVVRFTR
181NGGNATLQVD NWPVNEHYPT GNTDNERFQM VKQKIPFKYN RPVEEWLQEK GRQLTIFNTQ
241AQIAIGGKDK GRLFQGQLSG LYYDGLKVLN MAAENNPNIK INGSVRLVGE VPSILGTTQT
301TSMPPEMSTT VMETTTTMAT TTTRKNRSTA SIQPTSDDLV SSAECSSDDE DFVECEPSTA
361NPTEPGIRRV PGASEVIRES SSTTGMVVGI VAAAALCILI LLYAMYKYRN RDEGSYQVDE
421TRNYISNSAQ SNGTLMKEKQ QSSKSGHKKQ KNKDREYYV
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

459 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.