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NRXN3 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

NRXN3 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all NRXN3 antibodies →
$725.00each
Catalog Number: LS-G116411-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human NRXN3 (NM_004796), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
C14orf60 | KIAA0743 | Neurexin III-beta | Neurexin 3 | Neurexin III | NRXN3 | Neurexin III-alpha | Neurexin-3-beta
Origin Species
Human
NCBI GenBank
NM_004796
SwissProt
Q9Y4C0

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
117.32 kDa
Protein Type
Lysate
Amino Acid Sequence
1MLGSDDFFYV GGSPSTADLP GSPVSNNFMG CLKEVVYKNN DIRLELSRLA RIADTKMKIY
61GEVVFKCENV ATLDPINFET PEAYISLPKW NTKRMGSISF DFRTTEPNGL ILFTHGKPQE
121RKDARSQKNT KVDFFAVELL DGNLYLLLDM GSGTIKVKAT QKKANDGEWY HVDIQRDGRS
181GTISVNSRRT PFTASGESEI LDLEGDMYLG GLPENRAGLI LPTELWTAML NYGYVGCIRD
241LFIDGRSKNI RQLAEMQNAA GVKSSCSRMS AKQCDSYPCK NNAVCKDGWN RFICDCTGTG
301YWGRTCEREA SILSYDGSMY MKIIMPMVMH TEAEDVSFRF MSQRAYGLLV ATTSRDSADT
361LRLELDGGRV KLMVNLDCIR INCNSSKGPE TLYAGQKLND NEWHTVRVVR RGKSLKLTVD
421DDVAEGTMVG DHTRLEFHNI ETGIMTEKRY ISVVPSSFIG HLQSLMFNGL LYIDLCKNGD
481IDYCELKARF GLRNIIADPV TFKTKSSYLS LATLQAYTSM HLFFQFKTTS PDGFILFNSG
541DGNDFIAVEL VKGYIHYVFD LGNGPNVIKG NSDRPLNDNQ WHNVVITRDN SNTHSLKVDT
601KVVTQVINGA KNLDLKGDLY MAGLAQGMYS NLPKLVASRD GFQGCLASVD LNGRLPDLIN
661DALHRSGQIE RGCEGPSTTC QEDSCANQGV CMQQWEGFTC DCSMTSYSGN QCNDPGATYI
721FGKSGGLILY TWPANDRPST RSDRLAVGFS TTVKDGILVR IDSAPGLGDF LQLHIEQGKI
781GVVFNIGTVD ISIKEERTPV NDGKYHVVRF TRNGGNATLQ VDNWPVNEHY PTGRQLTIFN
841TQAQIAIGGK DKGRLFQGQL SGLYYDGLKV LNMAAENNPN IKINGSVRLV GEVPSILGTT
901QTTSMPPEMS TTVMETTTTM ATTTTRKNRS TASIQPTSDD LVSSAECSSD DEDFVECEPS
961TANPTEPGIR RVPGASEVIR ESSSTTGMVV GIVAAAALCI LILLYAMYKY RNRDEGSYQV
1021DETRNYISNS AQSNGTLMKE KQQSSKSGHK KQKNKDREYY V
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1061 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.