Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
NRF1 / NRF-1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G120248-100
Catalog Number: LS-G120248
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human NRF1 (NM_001040110), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- EWG | NRF-1 | NRF1 | Nuclear respiratory factor 1 | ALPHA-PAL
- Origin Species
- Human
- NCBI GenBank
- NM_005011
- SwissProt
- Q16656
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 53.4 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MEEHGVTQTE HMATIEAHAV AQQVQQVHVA TYTEHSMLSA DEDSPSSPED TSYDDSDILN61STAADEVTAH LAAAGPVGMA AAAAVATGKK RKRPHVFESN PSIRKRQQTR LLRKLRATLD121EYTTRVGQQA IVLCISPSKP NPVFKVFGAA PLENVVRKYK SMILEDLESA LAEHAPAPQE181VNSELPPLTI DGIPVSVDKM TQAQLRAFIP EMLKYSTGRG KPGWGKESCK PIWWPEDIPW241ANVRSDVRTE EQKQRVSWTQ ALRTIVKNCY KQHGREDLLY AFEDQQTQTQ ATATHSIAHL301VPSQTVVQTF SNPDGTVSLI QVGTGATVAT LADASELPTT VTVAQVNYSA VADGEVEQNW361ATLQGGEMTI QTTQASEATQ AVASLAEAAV AASQEMQQGA TVTMALNSEA AAHAVATLAE421ATLQGGGQIV LSGETAAAVG ALTGVQDANG LVQIPVSMYQ TVVTSLAQGN GPVQVAMAPV481TTRISDSAVT MDGQAVEVVT LEQTRTRPLEQKLISEEDLAANDILDYKDDDDKV
503 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.