NRF1 / NRF-1 Antibody
Catalog Number: LS-C761191
4 sizes / from $261.00
Antibody: Rabbit Polyclonal
Applications: Western Blot
Reactivity: Human, Mouse, Zebrafish
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human NRF1 / NRF-1 Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$220.00each
Catalog Number: LS-G77764-20
Catalog Number: LS-G77764
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human NRF1 (NM_001040110) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- EWG | NRF-1 | NRF1 | Nuclear respiratory factor 1 | ALPHA-PAL
- Origin Species
- Human
- NCBI GenBank
- NM_005011
- SwissProt
- Q16656
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 53.4 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MEEHGVTQTE HMATIEAHAV AQQVQQVHVA TYTEHSMLSA DEDSPSSPED TSYDDSDILN61STAADEVTAH LAAAGPVGMA AAAAVATGKK RKRPHVFESN PSIRKRQQTR LLRKLRATLD121EYTTRVGQQA IVLCISPSKP NPVFKVFGAA PLENVVRKYK SMILEDLESA LAEHAPAPQE181VNSELPPLTI DGIPVSVDKM TQAQLRAFIP EMLKYSTGRG KPGWGKESCK PIWWPEDIPW241ANVRSDVRTE EQKQRVSWTQ ALRTIVKNCY KQHGREDLLY AFEDQQTQTQ ATATHSIAHL301VPSQTVVQTF SNPDGTVSLI QVGTGATVAT LADASELPTT VTVAQVNYSA VADGEVEQNW361ATLQGGEMTI QTTQASEATQ AVASLAEAAV AASQEMQQGA TVTMALNSEA AAHAVATLAE421ATLQGGGQIV LSGETAAAVG ALTGVQDANG LVQIPVSMYQ TVVTSLAQGN GPVQVAMAPV481TTRISDSAVT MDGQAVEVVT LEQTRTRPLEQKLISEEDLAANDILDYKDDDDKV
503 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
