Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
NR2C2 / TAK1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G100021-100
Catalog Number: LS-G100021
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human NR2C2 (NM_003298), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- NR2C2 | Nuclear hormone receptor TR4 | Nuclear receptor TAK1 | Orphan nuclear receptor TR4 | Orphan nuclear receptor TAK1 | Testicular orphan receptor-4 | TR2R1 | TR4 | Testicular nuclear receptor 4 | Testicular receptor 4 | TR4 nuclear hormone receptor | HTAK1 | Nuclear receptor tr4 | TAK1
- Origin Species
- Human
- NCBI GenBank
- NM_003298
- SwissProt
- P49116
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 67.2 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MTSPSPRIQI ISTDSAVASP QRIQGSEPAS GPLSVFTSLN KEKIVTDQQT GQKIQIVTAV61DASGSPKQQF ILTSPDGAGT GKVILASPET SSAKQLIFTT SDNLVPGRIQ IVTDSASVER121LLGKTDVQRP QVVEYCVVCG DKASGRHYGA VSCEGCKGFF KRSVRKNLTY SCRSNQDCII181NKHHRNRCQF CRLKKCLEMG MKMESVQSER KPFDVQREKP SNCAASTEKI YIRKDLRSPL241IATPTFVADK DGARQTGLLD PGMLVNIQQP LIREDGTVLL ATDSKAETSQ GALGTLANVV301TSLANLSESL NNGDTSEIQP EDQSASEITR AFDTLAKALN TTDSSSSPSL ADGIDTSGGG361SIHVISRDQS TPIIEVEGPL LSDTHVTFKL TMPSPMPEYL NVHYICESAS RLLFLSMHWA421RSIPAFQALG QDCNTSLVRA CWNELFTLGL AQCAQVMSLS TILAAIVNHL QNSIQEDKLS481GDRIKQVMEH IWKLQEFCNS MAKLDIDGYE YAYLKAIVLF SPDHPGLTST SQIEKFQEKA541QMELQDYVQK TYSEDTYRLA RILVRLPALR LMSSNITEEL FFTGLIGNVS IDSIIPYILK601METAEYNGQI TGASLTRTRPLEQKLISEEDLAANDILDYKDDDDKV
615 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.