LSBio
Human NR2C2 / TAK1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human NR2C2 / TAK1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all NR2C2 antibodies →
$220.00each
Catalog Number: LS-G77837-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human NR2C2 (NM_003298) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
NR2C2 | Nuclear hormone receptor TR4 | Nuclear receptor TAK1 | Orphan nuclear receptor TR4 | Orphan nuclear receptor TAK1 | Testicular orphan receptor-4 | TR2R1 | TR4 | Testicular nuclear receptor 4 | Testicular receptor 4 | TR4 nuclear hormone receptor | HTAK1 | Nuclear receptor tr4 | TAK1
Origin Species
Human
NCBI GenBank
NM_003298
SwissProt
P49116

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
67.2 kDa
Protein Type
Lysate
Amino Acid Sequence
1MTSPSPRIQI ISTDSAVASP QRIQGSEPAS GPLSVFTSLN KEKIVTDQQT GQKIQIVTAV
61DASGSPKQQF ILTSPDGAGT GKVILASPET SSAKQLIFTT SDNLVPGRIQ IVTDSASVER
121LLGKTDVQRP QVVEYCVVCG DKASGRHYGA VSCEGCKGFF KRSVRKNLTY SCRSNQDCII
181NKHHRNRCQF CRLKKCLEMG MKMESVQSER KPFDVQREKP SNCAASTEKI YIRKDLRSPL
241IATPTFVADK DGARQTGLLD PGMLVNIQQP LIREDGTVLL ATDSKAETSQ GALGTLANVV
301TSLANLSESL NNGDTSEIQP EDQSASEITR AFDTLAKALN TTDSSSSPSL ADGIDTSGGG
361SIHVISRDQS TPIIEVEGPL LSDTHVTFKL TMPSPMPEYL NVHYICESAS RLLFLSMHWA
421RSIPAFQALG QDCNTSLVRA CWNELFTLGL AQCAQVMSLS TILAAIVNHL QNSIQEDKLS
481GDRIKQVMEH IWKLQEFCNS MAKLDIDGYE YAYLKAIVLF SPDHPGLTST SQIEKFQEKA
541QMELQDYVQK TYSEDTYRLA RILVRLPALR LMSSNITEEL FFTGLIGNVS IDSIIPYILK
601METAEYNGQI TGASL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

615 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.