Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
NR1I3 / CAR Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G120006-100
Catalog Number: LS-G120006
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Constitutive androstane receptor(NR1I3) (NM_001077479), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Car-beta | CAR1 | Constitutive active receptor | Constitutive active response | NR1I3 | Orphan nuclear receptor MB67 | MB67 | CAR
- Origin Species
- Human
- NCBI GenBank
- NM_005122
- SwissProt
- Q14994
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 36.2 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MLPKRSRRTV SKSIGPTCPF AGSCEVSKTQ RRHCPACRLQ KCLDAGMRKD MILSAEALAL61RRAKQAQRRA QQTPVQLSKE QEELIRTLLG AHTRHMGTMF EQFVQFRPPA HLFIHHQPLP121TLAPVLPLVT HFADINTFMV LQVIKFTKDL PVFRSLPIED QISLLKGAAV EICHIVLNTT181FCLQTQNFLC GPLRYTIEDG ARVGFQVEFL ELLFHFHGTL RKLQLQEPEY VLLAAMALFS241PDRPGVTQRD EIDQLQEEMA LTLQSYIKGQ QRRPRDRFLY AKLLGLLAEL RSINEAYGYQ301IQHIQGLSAM MPLLQEICSTRTRPLEQKLISEEDLAANDILDYKDDDDKV
319 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.