Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
NR1I3 / CAR Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G120000-100
Catalog Number: LS-G120000
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Constitutive androstane receptor(NR1I3) (NM_001077475), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Car-beta | CAR1 | Constitutive active receptor | Constitutive active response | NR1I3 | Orphan nuclear receptor MB67 | MB67 | CAR
- Origin Species
- Human
- NCBI GenBank
- NM_005122
- SwissProt
- Q14994
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 30.1 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MLPKRSRRTV SKSIGPTCPF AGSCEVSKTQ RRHCPACRLQ KCLDAGMRKD MILSAEALAL61RRAKQAQRRA QQTPVQLSKE QEELIRTLLG AHTRHMGTMF EQFVQFRPPA HLFIHHQPLP121TLAPVLPLVT HFADINTFMV LQVIKFTKDL PVFRSLPIED QISLLKGAAV EICHIVLNTT181FCLQTQNFLC GPLRYTIEDG ARDRPGVTQR DEIDQLQEEM ALTLQSYIKG QQRRPRDRSP241GTPWIHWSGK MLGPKIGPGS KGAQWLQTRTRPLEQKLISEEDLAANDILDYKDDDDKV
267 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.