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NPTX1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

NPTX1 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G117929-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human NPTX1 (NM_002522), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Neuronal pentraxin-1 | NPTX1 | Neuronal pentraxin I | NP-I | NP1
Origin Species
Human
NCBI GenBank
NM_002522
SwissProt
Q15818

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
47.12 kDa
Protein Type
Lysate
Amino Acid Sequence
1MPAGRAARTC ALLALCLLGA GAQDFGPTRF ICTSVPVDAD MCAASVAAGG AEELRSSVLQ
61LRETVLQQKE TILSQKETIR ELTAKLGRCE SQSTLDPGAG EARAGGGRKQ PGSGKNTMGD
121LSRTPAAETL SQLGQTLQSL KTRLENLEQY SRLNSSSQTN SLKDLLQSKI DELERQVLSR
181VNTLEEGKGG PRNDTEERVK IETALTSLHQ RISELEKGQK DNRPGDKFQL TFPLRTNYMY
241AKVKKSLPEM YAFTVCMWLK SSATPGVGTP FSYAVPGQAN ELVLIEWGNN PMEILINDKV
301AKLPFVINDG KWHHICVTWT TRDGVWEAYQ DGTQGGSGEN LAPYHPIKPQ GVLVLGQEQD
361TLGGGFDATQ AFVGELAHFN IWDRKLTPGE VYNLATCSTK ALSGNVIAWA ESHIEIYGGA
421TKWTFEACRQ IN
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

432 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.