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NOVA1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

NOVA1 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G115271-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human NOVA1 (NM_006489), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Nova-1 | Onconeural ventral antigen 1 | RNA-binding protein Nova-1 | NOVA1 | Paraneoplastic Ri antigen
Origin Species
Human
NCBI GenBank
NM_002515
SwissProt
P51513

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
49.1 kDa
Protein Type
Lysate
Amino Acid Sequence
1MMAAAPIQQN GTHTGVPIDL DPPDSRKRPL EAPPEAGSTK RTNTGEDGQY FLKVLIPSYA
61AGSIIGKGGQ TIVQLQKETG ATIKLSKSKD FYPGTTERVC LIQGTVEALN AVHGFIAEKI
121REMPQNVAKT EPVSILQPQT TVNPDRIKQV KIIVPNSTAG LIIGKGGATV KAVMEQSGAW
181VQLSQKPDGI NLQERVVTVS GEPEQNRKAV ELIIQKIQED PQSGSCLNIS YANVTGPVAN
241SNPTGSPYAN TAEVLPTAAA AAGLLGHANL AGVAAFPAVL SGFTGNDLVA ITSALNTLAS
301YGYNLNTLGL GLSQAAATGA LAAAAASANP AAAAANLLAT YASEASASGS TAGGTAGTFA
361LGSLAAATAA TNGYFGAASP LAASAILGTE KSTDGSKDVV EIAVPENLVG AILGKGGKTL
421VEYQELTGAR IQISKKGEFV PGTRNRKVTI TGTPAATQAA QYLITQRITY EQGVRAANPQ
481KVG
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

483 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.