Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
NOP2 / NOL1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G120961-100
Catalog Number: LS-G120961
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human NOP2 (NM_001033714), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Origin Species
- Human
- NCBI GenBank
- NM_006170
- SwissProt
- P46087
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 88.8 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MGRKLDPTKE KRGPGRKARK QKGAETELVR FLPAVSDENS KRLSSRARKR AAKRRLGSVE61APKTNKSPEA KPLPGKLPKG AVQTAGKKGP QSLFNAPRGK KRPAPGSDEE EEEEDSEEDG121MVNHGDLWGS EDDADTVDDY GADSNSEDEE EGEALLPIER AARKQKAREA AAGIQWSEEE181TEDEEEEKEV TPESGPPKVE EADGGLQINV DEEPFVLPPA GEMEQDAQAP DLQRVHKRIQ241DIVGILRDFG AQREEGRSRS EYLNRLKKDL AIYYSYGDFL LGKLMDLFPL SELVEFLEAN301EVPRPVTLRT NTLKTRRRDL AQALINRGVN LDPLGKWSKT GLVVYDSSVP IGATPEYLAG361HYMLQGASSM LPVMALAPQE HERILDMCCA PGGKTSYMAQ LMKNTGVILA NDANAERLKS421VVGNLHRLGV TNTIISHYDG RQFPKVVGGF DRVLLDAPCS GTGVISKDPA VKTNKDEKDI481LRCAHLQKEL LLSAIDSVNA TSKTGGYLVY CTCSITVEEN EWVVDYALKK RNVRLVPTGL541DFGQEGFTRF RERRFHPSLR STRRFYPHTH NMDGFFIAKF KKFSNSIPQS QTGNSETATP601TNVDLPQVIP KSENSSQPAK KAKGAAKTKQ QLQKQQHPKK ASFQKLNGIS KGADSELSTV661PSVTKTQASS SFQDSSQPAG KAEGIREPKV TGKLKQRSPK LQSSKKVAFL RQNAPPKGTD721TQTPAVLSPS KTQATLKPKD HHQPLGRAKG VEKQQLPEQP FEKAAFQKQN DTPKGPQPPT781VSPIRSSRPP PAKRKKSQSR GNSQLLLSTRTRPLEQKLISEEDLAANDILDYKDDDDKV
808 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.