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Human NOP2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human NOP2 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all NOP2 antibodies →
$220.00each
Catalog Number: LS-G93122-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human NOP2 (NM_001033714) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
NOP2 | Nop2p | p120 | NOP120 | YNA1 | NOL1 | NSUN1
Origin Species
Human
SwissProt
P40991

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
88.8 kDa
Protein Type
Lysate
Amino Acid Sequence
1MGRKLDPTKE KRGPGRKARK QKGAETELVR FLPAVSDENS KRLSSRARKR AAKRRLGSVE
61APKTNKSPEA KPLPGKLPKG AVQTAGKKGP QSLFNAPRGK KRPAPGSDEE EEEEDSEEDG
121MVNHGDLWGS EDDADTVDDY GADSNSEDEE EGEALLPIER AARKQKAREA AAGIQWSEEE
181TEDEEEEKEV TPESGPPKVE EADGGLQINV DEEPFVLPPA GEMEQDAQAP DLQRVHKRIQ
241DIVGILRDFG AQREEGRSRS EYLNRLKKDL AIYYSYGDFL LGKLMDLFPL SELVEFLEAN
301EVPRPVTLRT NTLKTRRRDL AQALINRGVN LDPLGKWSKT GLVVYDSSVP IGATPEYLAG
361HYMLQGASSM LPVMALAPQE HERILDMCCA PGGKTSYMAQ LMKNTGVILA NDANAERLKS
421VVGNLHRLGV TNTIISHYDG RQFPKVVGGF DRVLLDAPCS GTGVISKDPA VKTNKDEKDI
481LRCAHLQKEL LLSAIDSVNA TSKTGGYLVY CTCSITVEEN EWVVDYALKK RNVRLVPTGL
541DFGQEGFTRF RERRFHPSLR STRRFYPHTH NMDGFFIAKF KKFSNSIPQS QTGNSETATP
601TNVDLPQVIP KSENSSQPAK KAKGAAKTKQ QLQKQQHPKK ASFQKLNGIS KGADSELSTV
661PSVTKTQASS SFQDSSQPAG KAEGIREPKV TGKLKQRSPK LQSSKKVAFL RQNAPPKGTD
721TQTPAVLSPS KTQATLKPKD HHQPLGRAKG VEKQQLPEQP FEKAAFQKQN DTPKGPQPPT
781VSPIRSSRPP PAKRKKSQSR GNSQLLLS
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

808 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.