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NOL4 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

NOL4 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G131033-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human NOL4 (NM_001198547), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Cancer/testis antigen 125 | CT125 | HRIHFB2255 | Nucleolar-localized protein | NOL4 | NOLP | Nucleolar localized protein | Nucleolar protein 4
Origin Species
Human
NCBI GenBank
NM_003787
SwissProt
O94818

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
63.4 kDa
Protein Type
Lysate
Amino Acid Sequence
1MADLMQETFL HHATDGVGVD EKLSLRRVAV VEDFFDIIYS MHVETGPNGE QIRKHAGQKR
61TYKAISESYA FLPREAVTRF LMSCSECQKR MHLNPDGTDH KDNGKPPTLV TSMIDYNMPI
121TMAYMKHMKL QLLNSQQDED ESSIESDEFD MSDSTRMSAV NSDLSSNLEE RMQSPQNLHG
181QQDDDSAAES FNGNETLGHS SIASGGTHSR EMGDSNSDGK TGLEQDEQPL NLSDSPLSAQ
241LTSEYRIDDH NSNGKNKYKN LLISDLKMER EARENGSKSP AHSYSSYDSG KNESVDRGAE
301DLSLNRGDED EDDHEDHDDS EKVNETDGVE AERLKAFNMF VRLFVDENLD RMVPISKQPK
361EKIQAIIDSC RRQFPEYQER ARKRIRTYLK SCRRMKRSGF EMSRPIPSHL TSAVAESILA
421SACESESRNA AKRMRLERQQ DESAPADKQC KPEATQATYS TSAVPGSQDV LYINGNGTYS
481YHSYRGLGGG LLNLNDASSS GPTDLSMKRQ LATSSGSSSS SNSRPQLSPT EINAVRQLVA
541GYRESAAFLL RSADELENLI LQQN
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

564 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.