NOL4 Antibody
Catalog Number: LS-C808150
2 sizes / from $288.00
Antibody: Rabbit Polyclonal
Applications: Enzyme-Linked Immunosorbent Assay, Western Blot
Reactivity: Human, Mouse, Rat
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human NOL4 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$153.00each
Catalog Number: LS-G86388-20
Catalog Number: LS-G86388
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human NOL4 (NM_001198547) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Cancer/testis antigen 125 | CT125 | HRIHFB2255 | Nucleolar-localized protein | NOL4 | NOLP | Nucleolar localized protein | Nucleolar protein 4
- Origin Species
- Human
- NCBI GenBank
- NM_003787
- SwissProt
- O94818
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 63.4 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MADLMQETFL HHATDGVGVD EKLSLRRVAV VEDFFDIIYS MHVETGPNGE QIRKHAGQKR61TYKAISESYA FLPREAVTRF LMSCSECQKR MHLNPDGTDH KDNGKPPTLV TSMIDYNMPI121TMAYMKHMKL QLLNSQQDED ESSIESDEFD MSDSTRMSAV NSDLSSNLEE RMQSPQNLHG181QQDDDSAAES FNGNETLGHS SIASGGTHSR EMGDSNSDGK TGLEQDEQPL NLSDSPLSAQ241LTSEYRIDDH NSNGKNKYKN LLISDLKMER EARENGSKSP AHSYSSYDSG KNESVDRGAE301DLSLNRGDED EDDHEDHDDS EKVNETDGVE AERLKAFNMF VRLFVDENLD RMVPISKQPK361EKIQAIIDSC RRQFPEYQER ARKRIRTYLK SCRRMKRSGF EMSRPIPSHL TSAVAESILA421SACESESRNA AKRMRLERQQ DESAPADKQC KPEATQATYS TSAVPGSQDV LYINGNGTYS481YHSYRGLGGG LLNLNDASSS GPTDLSMKRQ LATSSGSSSS SNSRPQLSPT EINAVRQLVA541GYRESAAFLL RSADELENLI LQQNTRTRPLEQKLISEEDLAANDILDYKDDDDKV
564 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
