Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
NOD3 / NLRC3 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G104606-100
Catalog Number: LS-G104606
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human NLRC3 (NM_178844), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- CARD15-like protein | Caterpiller 16.2 | Caterpiller protein 16.2 | NLRC3 | NOD-like receptor C3 | NOD3 | Protein NLRC3 | CLR16.2
- Origin Species
- Human
- SwissProt
- Q7RTR2
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 114.5 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MRKQEVRTGR EAGQGHGTGS PAEQVKALMD LLAGKGSQGS QAPQALDRTP DAPLGPCSND61SRIQRHRKAL LSKVGGGPEL GGPWHRLASL LLVEGLTDLQ LREHDFTQVE ATRGGGHPAR121TVALDRLFLP LSRVSVPPRV SITIGVAGMG KTTLVRHFVR LWAHGQVGKD FSLVLPLTFR181DLNTHEKLCA DRLICSVFPH VGEPSLAVAV PARALLILDG LDECRTPLDF SNTVACTDPK241KEIPVDHLIT NIIRGNLFPE VSIWITSRPS ASGQIPGGLV DRMTEIRGFN EEEIKVCLEQ301MFPEDQALLG WMLSQVQADR ALYLMCTVPA FCRLTGMALG HLWRSRTGPQ DAELWPPRTL361CELYSWYFRM ALSGEGQEKG KASPRIEQVA HGGRKMVGTL GRLAFHGLLK KKYVFYEQDM421KAFGVDLALL QGAPCSCFLQ REETLASSVA YCFTHLSLQE FVAAAYYYGA SRRAIFDLFT481ESGVSWPRLG FLTHFRSAAQ RAMQAEDGRL DVFLRFLSGL LSPRVNALLA GSLLAQGEHQ541AYRTQVAELL QGCLRPDAAV CARAINVLHC LHELQHTELA RSVEEAMESG ALARLTGPAH601RAALAYLLQV SDACAQEANL SLSLSQGVLQ SLLPQLLYCR KLRLDTNQFQ DPVMELLGSV661LSGKDCRIQK ISLAENQISN KGAKALARSL LVNRSLTSLD LRGNSIGPQG AKALADALKI721NRTLTSLSLQ GNTVRDDGAR SMAEALASNR TLSMLHLQKN SIGPMGAQRM ADALKQNRSL781KELMFSSNSI GDGGAKALAE ALKVNQGLES LDLQSNSISD AGVAALMGAL CTNQTLLSLS841LRENSISPEG AQAIAHALCA NSTLKNLDLT ANLLHDQGAR AIAVAVRENR TLTSLHLQWN901FIQAGAAQAL GQALQLNRSL TSLDLQENAI GDDGACAVAR ALKVNTALTA LYLQVASIGA961SGAQVLGEAL AVNRTLEILD LRGNAIGVAG AKALANALKV NSSLRRLNLQ ENSLGMDGAI1021CIATALSGNH RLQHINLQGN HIGDSGARMI SEAIKTNAPT CTVEMTRTRPLEQKLISEEDLAANDILDYKDDDDKV
1065 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.