LSBio
Human NOD3 / NLRC3 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human NOD3 / NLRC3 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all NLRC3 antibodies →
$220.00each
Catalog Number: LS-G94177-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human NLRC3 (NM_178844) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
CARD15-like protein | Caterpiller 16.2 | Caterpiller protein 16.2 | NLRC3 | NOD-like receptor C3 | NOD3 | Protein NLRC3 | CLR16.2
Origin Species
Human
SwissProt
Q7RTR2

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
114.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MRKQEVRTGR EAGQGHGTGS PAEQVKALMD LLAGKGSQGS QAPQALDRTP DAPLGPCSND
61SRIQRHRKAL LSKVGGGPEL GGPWHRLASL LLVEGLTDLQ LREHDFTQVE ATRGGGHPAR
121TVALDRLFLP LSRVSVPPRV SITIGVAGMG KTTLVRHFVR LWAHGQVGKD FSLVLPLTFR
181DLNTHEKLCA DRLICSVFPH VGEPSLAVAV PARALLILDG LDECRTPLDF SNTVACTDPK
241KEIPVDHLIT NIIRGNLFPE VSIWITSRPS ASGQIPGGLV DRMTEIRGFN EEEIKVCLEQ
301MFPEDQALLG WMLSQVQADR ALYLMCTVPA FCRLTGMALG HLWRSRTGPQ DAELWPPRTL
361CELYSWYFRM ALSGEGQEKG KASPRIEQVA HGGRKMVGTL GRLAFHGLLK KKYVFYEQDM
421KAFGVDLALL QGAPCSCFLQ REETLASSVA YCFTHLSLQE FVAAAYYYGA SRRAIFDLFT
481ESGVSWPRLG FLTHFRSAAQ RAMQAEDGRL DVFLRFLSGL LSPRVNALLA GSLLAQGEHQ
541AYRTQVAELL QGCLRPDAAV CARAINVLHC LHELQHTELA RSVEEAMESG ALARLTGPAH
601RAALAYLLQV SDACAQEANL SLSLSQGVLQ SLLPQLLYCR KLRLDTNQFQ DPVMELLGSV
661LSGKDCRIQK ISLAENQISN KGAKALARSL LVNRSLTSLD LRGNSIGPQG AKALADALKI
721NRTLTSLSLQ GNTVRDDGAR SMAEALASNR TLSMLHLQKN SIGPMGAQRM ADALKQNRSL
781KELMFSSNSI GDGGAKALAE ALKVNQGLES LDLQSNSISD AGVAALMGAL CTNQTLLSLS
841LRENSISPEG AQAIAHALCA NSTLKNLDLT ANLLHDQGAR AIAVAVRENR TLTSLHLQWN
901FIQAGAAQAL GQALQLNRSL TSLDLQENAI GDDGACAVAR ALKVNTALTA LYLQVASIGA
961SGAQVLGEAL AVNRTLEILD LRGNAIGVAG AKALANALKV NSSLRRLNLQ ENSLGMDGAI
1021CIATALSGNH RLQHINLQGN HIGDSGARMI SEAIKTNAPT CTVEM
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1065 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.