Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
NLRP7 / NALP7 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G107090-100
Catalog Number: LS-G107090
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human NLRP7 (NM_139176), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- CLR19.4 | NALP7 | NOD12 | NLRP7 | PYPAF3 | PAN7 | HYDM
- Origin Species
- Human
- SwissProt
- Q8WX94
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 114.7 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MTSPQLEWTL QTLLEQLNED ELKSFKSLLW AFPLEDVLQK TPWSEVEEAD GKKLAEILVN61TSSENWIRNA TVNILEEMNL TELCKMAKAE MMEDGQVQEI DNPELGDAEE DSELAKPGEK121EGWRNSMEKQ SLVWKNTFWQ GDIDNFHDDV TLRNQRFIPF LNPRTPRKLT PYTVVLHGPA181GVGKTTLAKK CMLDWTDCNL SPTLRYAFYL SCKELSRMGP CSFAELISKD WPELQDDIPS241ILAQAQRILF VVDGLDELKV PPGALIQDIC GDWEKKKPVP VLLGSLLKRK MLPRAALLVT301TRPRALRDLQ LLAQQPIYVR VEGFLEEDRR AYFLRHFGDE DQAMRAFELM RSNAALFQLG361SAPAVCWIVC TTLKLQMEKG EDPVPTCLTR TGLFLRFLCS RFPQGAQLRG ALRTLSLLAA421QGLWAQMSVF HREDLERLGV QESDLRLFLD GDILRQDRVS KGCYSFIHLS FQQFLTALFY481ALEKEEGEDR DGHAWDIGDV QKLLSGEERL KNPDLIQVGH FLFGLANEKR AKELEATFGC541RMSPDIKQEL LQCKAHLHAN KPLSVTDLKE VLGCLYESQE EELAKVVVAP FKEISIHLTN601TSEVMHCSFS LKHCQDLQKL SLQVAKGVFL ENYMDFELDI EFESSNSNLK FLEVKQSFLS661DSSVRILCDH VTRSTCHLQK VEIKNVTPDT AYRDFCLAFI GKKTLTHLTL AGHIEWERTM721MLMLCDLLRN HKCNLQYLRL GGHCATPEQW AEFFYVLKAN QSLKHLRLSA NVLLDEGAML781LYKTMTRPKH FLQMLSLENC RLTEASCKDL AAVLVVSKKL THLCLAKNPI GDTGVKFLCE841GLSYPDCKLQ TLVLQQCSIT KLGCRYLSEA LQEACSLTNL DLSINQIARG LWILCQALEN901PNCNLKHLRL WSCSLMPFYC QHLGSALLSN QKLETLDLGQ NHLWKSGIIK LFGVLRQRTG961SLKILRLKTY ETNLEIKKLL EEVKEKNPKL TIDCNASGAT APPCCDFFCSGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
1009 aa + 34 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.