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Human NLRP7 / NALP7 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human NLRP7 / NALP7 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all NLRP7 antibodies →
$220.00each
Catalog Number: LS-G94171-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human NLRP7 (NM_139176) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
CLR19.4 | NALP7 | NOD12 | NLRP7 | PYPAF3 | PAN7 | HYDM
Origin Species
Human
SwissProt
Q8WX94

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
114.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MTSPQLEWTL QTLLEQLNED ELKSFKSLLW AFPLEDVLQK TPWSEVEEAD GKKLAEILVN
61TSSENWIRNA TVNILEEMNL TELCKMAKAE MMEDGQVQEI DNPELGDAEE DSELAKPGEK
121EGWRNSMEKQ SLVWKNTFWQ GDIDNFHDDV TLRNQRFIPF LNPRTPRKLT PYTVVLHGPA
181GVGKTTLAKK CMLDWTDCNL SPTLRYAFYL SCKELSRMGP CSFAELISKD WPELQDDIPS
241ILAQAQRILF VVDGLDELKV PPGALIQDIC GDWEKKKPVP VLLGSLLKRK MLPRAALLVT
301TRPRALRDLQ LLAQQPIYVR VEGFLEEDRR AYFLRHFGDE DQAMRAFELM RSNAALFQLG
361SAPAVCWIVC TTLKLQMEKG EDPVPTCLTR TGLFLRFLCS RFPQGAQLRG ALRTLSLLAA
421QGLWAQMSVF HREDLERLGV QESDLRLFLD GDILRQDRVS KGCYSFIHLS FQQFLTALFY
481ALEKEEGEDR DGHAWDIGDV QKLLSGEERL KNPDLIQVGH FLFGLANEKR AKELEATFGC
541RMSPDIKQEL LQCKAHLHAN KPLSVTDLKE VLGCLYESQE EELAKVVVAP FKEISIHLTN
601TSEVMHCSFS LKHCQDLQKL SLQVAKGVFL ENYMDFELDI EFESSNSNLK FLEVKQSFLS
661DSSVRILCDH VTRSTCHLQK VEIKNVTPDT AYRDFCLAFI GKKTLTHLTL AGHIEWERTM
721MLMLCDLLRN HKCNLQYLRL GGHCATPEQW AEFFYVLKAN QSLKHLRLSA NVLLDEGAML
781LYKTMTRPKH FLQMLSLENC RLTEASCKDL AAVLVVSKKL THLCLAKNPI GDTGVKFLCE
841GLSYPDCKLQ TLVLQQCSIT KLGCRYLSEA LQEACSLTNL DLSINQIARG LWILCQALEN
901PNCNLKHLRL WSCSLMPFYC QHLGSALLSN QKLETLDLGQ NHLWKSGIIK LFGVLRQRTG
961SLKILRLKTY ETNLEIKKLL EEVKEKNPKL TIDCNASGAT APPCCDFFC
SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV

1009 aa + 34 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

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