NLRP5 / NALP5 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G102395-100
Catalog Number: LS-G102395
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human NALP5(NLRP5) (NM_153447), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- CLR19.8 | MATER | NLRP5 | PAN11 | NALP5 | PYPAF8 | Mater protein homolog
- Origin Species
- Human
- NCBI GenBank
- NM_153447
- SwissProt
- P59047
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 134.8 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MKVAGGLELG AAALLSASPR ALVTLSTGPT CSILPKNPLF PQNLSSQPCI KMEGDKSLTF61SSYGLQWCLY ELDKEEFQTF KELLKKKSSE STTCSIPQFE IENANVECLA LLLHEYYGAS121LAWATSISIF ENMNLRTLSE KARDDMKRHS PEDPEATMTD QGPSKEKVPG ISQAVQQDSA181TAAETKEQEI SQAMEQEGAT AAETEEQEIS QAMEQEGATA AETEEQGHGG DTWDYKSHVM241TKFAEEEDVR RSFENTAADW PEMQTLAGAF DSDRWGFRPR TVVLHGKSGI GKSALARRIV301LCWAQGGLYQ GMFSYVFFLP VREMQRKKES SVTEFISREW PDSQAPVTEI MSRPERLLFI361IDGFDDLGSV LNNDTKLCKD WAEKQPPFTL IRSLLRKVLL PESFLIVTVR DVGTEKLKSE421VVSPRYLLVR GISGEQRIHL LLERGIGEHQ KTQGLRAIMN NRELLDQCQV PAVGSLICVA481LQLQDVVGES VAPFNQTLTG LHAAFVFHQL TPRGVVRRCL NLEERVVLKR FCRMAVEGVW541NRKSVFDGDD LMVQGLGESE LRALFHMNIL LPDSHCEEYY TFFHLSLQDF CAALYYVLEG601LEIEPALCPL YVEKTKRSME LKQAGFHIHS LWMKRFLFGL VSEDVRRPLE VLLGCPVPLG661VKQKLLHWVS LLGQQPNATT PGDTLDAFHC LFETQDKEFV RLALNSFQEV WLPINQNLDL721IASSFCLQHC PYLRKIRVDV KGIFPRDESA EACPVVPLWM RDKTLIEEQW EDFCSMLGTH781PHLRQLDLGS SILTERAMKT LCAKLRHPTC KIQTLMFRNA QITPGVQHLW RIVMANRNLR841SLNLGGTHLK EEDVRMACEA LKHPKCLLES LRLDCCGLTH ACYLKISQIL TTSPSLKSLS901LAGNKVTDQG VMPLSDALRV SQCALQKLIL EDCGITATGC QSLASALVSN RSLTHLCLSN961NSLGNEGVNL LCRSMRLPHC SLQRLMLNQC HLDTAGCGFL ALALMGNSWL THLSLSMNPV1021EDNGVKLLCE VMREPSCHLQ DLELVKCHLT AACCESLSCV ISRSRHLKSL DLTDNALGDG1081GVAALCEGLK QKNSVLARLG LKACGLTSDC CEALSLALSC NRHLTSLNLV QNNFSPKGMM1141KLCSAFACPT SNLQIIGLWK WQYPVQIRKL LEEVQLLKPR VVIDGSWHSF DEDDRYWWKNTRTRPLEQKLISEEDLAANDILDYKDDDDKV
1200 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.