LSBio
Human NLRP5 / NALP5 Protein (Over-Expression Lysate Myc-DDK (Flag))

Human NLRP5 / NALP5 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
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$220.00each
Catalog Number: LS-G94139-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human NALP5(NLRP5) (NM_153447) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
CLR19.8 | MATER | NLRP5 | PAN11 | NALP5 | PYPAF8 | Mater protein homolog
Origin Species
Human
NCBI GenBank
NM_153447
SwissProt
P59047

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
134.8 kDa
Protein Type
Lysate
Amino Acid Sequence
1MKVAGGLELG AAALLSASPR ALVTLSTGPT CSILPKNPLF PQNLSSQPCI KMEGDKSLTF
61SSYGLQWCLY ELDKEEFQTF KELLKKKSSE STTCSIPQFE IENANVECLA LLLHEYYGAS
121LAWATSISIF ENMNLRTLSE KARDDMKRHS PEDPEATMTD QGPSKEKVPG ISQAVQQDSA
181TAAETKEQEI SQAMEQEGAT AAETEEQEIS QAMEQEGATA AETEEQGHGG DTWDYKSHVM
241TKFAEEEDVR RSFENTAADW PEMQTLAGAF DSDRWGFRPR TVVLHGKSGI GKSALARRIV
301LCWAQGGLYQ GMFSYVFFLP VREMQRKKES SVTEFISREW PDSQAPVTEI MSRPERLLFI
361IDGFDDLGSV LNNDTKLCKD WAEKQPPFTL IRSLLRKVLL PESFLIVTVR DVGTEKLKSE
421VVSPRYLLVR GISGEQRIHL LLERGIGEHQ KTQGLRAIMN NRELLDQCQV PAVGSLICVA
481LQLQDVVGES VAPFNQTLTG LHAAFVFHQL TPRGVVRRCL NLEERVVLKR FCRMAVEGVW
541NRKSVFDGDD LMVQGLGESE LRALFHMNIL LPDSHCEEYY TFFHLSLQDF CAALYYVLEG
601LEIEPALCPL YVEKTKRSME LKQAGFHIHS LWMKRFLFGL VSEDVRRPLE VLLGCPVPLG
661VKQKLLHWVS LLGQQPNATT PGDTLDAFHC LFETQDKEFV RLALNSFQEV WLPINQNLDL
721IASSFCLQHC PYLRKIRVDV KGIFPRDESA EACPVVPLWM RDKTLIEEQW EDFCSMLGTH
781PHLRQLDLGS SILTERAMKT LCAKLRHPTC KIQTLMFRNA QITPGVQHLW RIVMANRNLR
841SLNLGGTHLK EEDVRMACEA LKHPKCLLES LRLDCCGLTH ACYLKISQIL TTSPSLKSLS
901LAGNKVTDQG VMPLSDALRV SQCALQKLIL EDCGITATGC QSLASALVSN RSLTHLCLSN
961NSLGNEGVNL LCRSMRLPHC SLQRLMLNQC HLDTAGCGFL ALALMGNSWL THLSLSMNPV
1021EDNGVKLLCE VMREPSCHLQ DLELVKCHLT AACCESLSCV ISRSRHLKSL DLTDNALGDG
1081GVAALCEGLK QKNSVLARLG LKACGLTSDC CEALSLALSC NRHLTSLNLV QNNFSPKGMM
1141KLCSAFACPT SNLQIIGLWK WQYPVQIRKL LEEVQLLKPR VVIDGSWHSF DEDDRYWWKN
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1200 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

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