Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
NLRC5 / NOD4 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G108966-100
Catalog Number: LS-G108966
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human NLRC5 (NM_032206), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- CLR16.1 | NOD-like receptor C5 | NOD27 | NLRC5 | NOD4 | Protein NLRC5 | Caterpiller protein 16.1
- Origin Species
- Human
- NCBI GenBank
- AK025212
- SwissProt
- Q86WI3
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 204.4 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MDPVGLQLGN KNLWSCLVRL LTKDPEWLNA KMKFFLPNTD LDSRNETLDP EQRVILQLNK61LHVQGSDTWQ SFIHCVCMQL EVPLDLEVLL LSTFGYDDGF TSQLGAEGKS QPESQLHHGL121KRPHQSCGSS PRRKQCKKQQ LELAKKYLQL LRTSAQQRYR SQIPGSGQPH AFHQVYVPPI181LRRATASLDT PEGAIMGDVK VEDGADVSIS DLFNTRVNKG PRVTVLLGKA GMGKTTLAHR241LCQKWAEGHL NCFQALFLFE FRQLNLITRF LTPSELLFDL YLSPESDHDT VFQYLEKNAD301QVLLIFDGLD EALQPMGPDG PGPVLTLFSH LCNGTLLPGC RVMATSRPGK LPACLPAEAA361MVHMLGFDGP RVEEYVNHFF SAQPSREGAL VELQTNGRLR SLCAVPALCQ VACLCLHHLL421PDHAPGQSVA LLPNMTQLYM QMVLALSPPG HLPTSSLLDL GEVALRGLET GKVIFYAKDI481APPLIAFGAT HSLLTSFCVC TGPGHQQTGY AFTHLSLQEF LAALHLMASP KVNKDTLTQY541VTLHSRWVQR TKARLGLSDH LPTFLAGLAS CTCRPFLSHL AQGNEDCVGA KQAAVVQVLK601KLATRKLTGP KVVELCHCVD ETQEPELASL TAQSLPYQLP FHNFPLTCTD LATLTNILEH661REAPIHLDFD GCPLEPHCPE ALVGCGQIEN LSFKSRKCGD AFAEALSRSL PTMGRLQMLG721LAGSKITARG ISHLVKALPL CPQLKEVSFR DNQLSDQVVL NIVEVLPHLP RLRKLDLSSN781SICVSTLLCL ARVAVTCPTV RMLQAREADL IFLLSPPTET TAELQRAPDL QESDGQRKGA841QSRSLTLRLQ KCQLQVRDAE ALIALLQEGP HLEEVDLSGN QLEDEGCRLM AEAASQLHIA901RKLDLSDNGL SVAGVHCVLR AVSACWTLAE LHISLQHKTV IFMFAQEPEE QKGPQERAAF961LDSLMLQMPS ELPLSSRRMR LTHCGLQEKH LEQLCKALGG SCHLGHLHLD FSGNALGDEG1021AARLAQLLPG LGALQSLNLS ENGLSLDAVL GLVRCFSTLQ WLFRLDISFE SQHILLRGDK1081TSRDMWATGS LPDFPAAAKF LGFRQRCIPR SLCLSECPLE PPSLTRLCAT LKDCPGPLEL1141QLSCEFLSDQ SLETLLDCLP QLPQLSLLQL SQTGLSPKSP FLLANTLSLC PRVKKVDLRS1201LHHATLHFRS NEEEEGVCCG RFTGCSLSQE HVESLCWLLS KCKDLSQVDL SANLLGDSGL1261RCLLECLPQV PISGLLDLSH NSISQESALY LLETLPSCPR VREASVNLGS EQSFRIHFSR1321EDQAGKTLRL SECSFRPEHV SRLATGLSKS LQLTELTLTQ CCLGQKQLAI LLSLVGRPAG1381LFSLRVQEPW ADRARVLSLL EVCAQASGSV TEISISETQQ QLCVQLEFPR QEENPEAVAL1441RLAHCDLGAH HSLLVGQLME TCARLQQLSL SQVNLCEDDD ASSLLLQSLL LSLSELKTFR1501LTSSCVSTEG LAHLASGLGH CHHLEELDLS NNQFDEEGTK ALMRALEGKW MLKRLDLSHL1561LLNSSTLALL THRLSQMTCL QSLRLNRNSI GDVGCCHLSE ALRAATSLEE LDLSHNQIGD1621AGVQHLATIL PGLPELRKID LSGNSISSAG GVQLAESLVL CRRLEELMLG CNALGDPTAL1681GLAQELPQHL RVLHLPFSHL GPGGALSLAQ ALDGSPHLEE ISLAENNLAG GVLRFCMELP1741LLRQIDLVSC KIDNQTAKLL TSSFTSCPAL EVILLSWNLL GDEAAAELAQ VLPQMGRLKR1801VDLEKNQITA LGAWLLAEGL AQGSSIQVIR LWNNPIPCDM AQHLKSQEPR LDFAFFDNQP1861QAPWGTTRTRPLEQKLISEEDLAANDILDYKDDDDKV
1866 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.