LSBio
Human NLRC5 / NOD4 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human NLRC5 / NOD4 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all NLRC5 antibodies →
$220.00each
Catalog Number: LS-G85823-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human NLRC5 (NM_032206) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
CLR16.1 | NOD-like receptor C5 | NOD27 | NLRC5 | NOD4 | Protein NLRC5 | Caterpiller protein 16.1
Origin Species
Human
NCBI GenBank
AK025212
SwissProt
Q86WI3

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
204.4 kDa
Protein Type
Lysate
Amino Acid Sequence
1MDPVGLQLGN KNLWSCLVRL LTKDPEWLNA KMKFFLPNTD LDSRNETLDP EQRVILQLNK
61LHVQGSDTWQ SFIHCVCMQL EVPLDLEVLL LSTFGYDDGF TSQLGAEGKS QPESQLHHGL
121KRPHQSCGSS PRRKQCKKQQ LELAKKYLQL LRTSAQQRYR SQIPGSGQPH AFHQVYVPPI
181LRRATASLDT PEGAIMGDVK VEDGADVSIS DLFNTRVNKG PRVTVLLGKA GMGKTTLAHR
241LCQKWAEGHL NCFQALFLFE FRQLNLITRF LTPSELLFDL YLSPESDHDT VFQYLEKNAD
301QVLLIFDGLD EALQPMGPDG PGPVLTLFSH LCNGTLLPGC RVMATSRPGK LPACLPAEAA
361MVHMLGFDGP RVEEYVNHFF SAQPSREGAL VELQTNGRLR SLCAVPALCQ VACLCLHHLL
421PDHAPGQSVA LLPNMTQLYM QMVLALSPPG HLPTSSLLDL GEVALRGLET GKVIFYAKDI
481APPLIAFGAT HSLLTSFCVC TGPGHQQTGY AFTHLSLQEF LAALHLMASP KVNKDTLTQY
541VTLHSRWVQR TKARLGLSDH LPTFLAGLAS CTCRPFLSHL AQGNEDCVGA KQAAVVQVLK
601KLATRKLTGP KVVELCHCVD ETQEPELASL TAQSLPYQLP FHNFPLTCTD LATLTNILEH
661REAPIHLDFD GCPLEPHCPE ALVGCGQIEN LSFKSRKCGD AFAEALSRSL PTMGRLQMLG
721LAGSKITARG ISHLVKALPL CPQLKEVSFR DNQLSDQVVL NIVEVLPHLP RLRKLDLSSN
781SICVSTLLCL ARVAVTCPTV RMLQAREADL IFLLSPPTET TAELQRAPDL QESDGQRKGA
841QSRSLTLRLQ KCQLQVRDAE ALIALLQEGP HLEEVDLSGN QLEDEGCRLM AEAASQLHIA
901RKLDLSDNGL SVAGVHCVLR AVSACWTLAE LHISLQHKTV IFMFAQEPEE QKGPQERAAF
961LDSLMLQMPS ELPLSSRRMR LTHCGLQEKH LEQLCKALGG SCHLGHLHLD FSGNALGDEG
1021AARLAQLLPG LGALQSLNLS ENGLSLDAVL GLVRCFSTLQ WLFRLDISFE SQHILLRGDK
1081TSRDMWATGS LPDFPAAAKF LGFRQRCIPR SLCLSECPLE PPSLTRLCAT LKDCPGPLEL
1141QLSCEFLSDQ SLETLLDCLP QLPQLSLLQL SQTGLSPKSP FLLANTLSLC PRVKKVDLRS
1201LHHATLHFRS NEEEEGVCCG RFTGCSLSQE HVESLCWLLS KCKDLSQVDL SANLLGDSGL
1261RCLLECLPQV PISGLLDLSH NSISQESALY LLETLPSCPR VREASVNLGS EQSFRIHFSR
1321EDQAGKTLRL SECSFRPEHV SRLATGLSKS LQLTELTLTQ CCLGQKQLAI LLSLVGRPAG
1381LFSLRVQEPW ADRARVLSLL EVCAQASGSV TEISISETQQ QLCVQLEFPR QEENPEAVAL
1441RLAHCDLGAH HSLLVGQLME TCARLQQLSL SQVNLCEDDD ASSLLLQSLL LSLSELKTFR
1501LTSSCVSTEG LAHLASGLGH CHHLEELDLS NNQFDEEGTK ALMRALEGKW MLKRLDLSHL
1561LLNSSTLALL THRLSQMTCL QSLRLNRNSI GDVGCCHLSE ALRAATSLEE LDLSHNQIGD
1621AGVQHLATIL PGLPELRKID LSGNSISSAG GVQLAESLVL CRRLEELMLG CNALGDPTAL
1681GLAQELPQHL RVLHLPFSHL GPGGALSLAQ ALDGSPHLEE ISLAENNLAG GVLRFCMELP
1741LLRQIDLVSC KIDNQTAKLL TSSFTSCPAL EVILLSWNLL GDEAAAELAQ VLPQMGRLKR
1801VDLEKNQITA LGAWLLAEGL AQGSSIQVIR LWNNPIPCDM AQHLKSQEPR LDFAFFDNQP
1861QAPWGT
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1866 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

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