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NLE1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

NLE1 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G111987-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human NLE1 (NM_018096), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
NLE1 | Notchless gene homolog | Nle | Notchless protein homolog 1
Origin Species
Human
NCBI GenBank
NM_018096
SwissProt
Q9NVX2

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
53.1 kda
Protein Type
Lysate
Amino Acid Sequence
1MAAAVADEAV ARDVQRLLVQ FQDEGGQLLG SPFDVPVDIT PDRLQLVCNA LLAQEDPLPL
61AFFVHDAEIV SSLGKTLESQ AVETEKVLDI IYQPQAIFRV RAVTRCTSSL EGHSEAVISV
121AFSPTGKYLA SGSGDTTVRF WDLSTETPHF TCKGHRHWVL SISWSPDGKK LASGCKNGQI
181LLWDPSTGKQ VGRTLAGHSK WITGLSWEPL HANPECRYVA SSSKDGSVRI WDTTAGRCER
241ILTGHTQSVT CLRWGGDGLL YSASQDRTIK VWRAHDGVLC RTLQGHGHWV NTMALSTDYA
301LRTGAFEPAE ASVNPQDLKG SLQELKERAL SRYNLVRGQG PERLVSGSDD FTLFLWSPAE
361DKKPLTRMTG HQALINQVLF SPDSRIVASA SFDKSIKLWD GRTGKYLASL RGHVAAVYQI
421AWSADSRLLV SGSSDSTLKV WDVKAQKLAM DLPGHADEVY AVDWSPDGQR VASGGKDKCL
481RIWRR
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

485 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.